Attachment V - Page 4 
4 
Dr. Pirone said he thought the document should require an extensive characteri- 
zation of the parent organism as the modified organism will retain the vast 
majority of the characteristics of the parent. Perhaps less information about 
the donor of the inserted DNA is required. However, should the transferred 
trait have a major ecological role more information on the function of the DNA 
donor may be required. Dr. Pirone pointed out that large numbers of modified 
organisms may be released. Dr. Vidaver said the characteristics of the recon- 
structed recipient are of greatest importance as the properties of the organism 
may be significantly altered. 
Dr. Lacy suggested item four might request a decription of the steps taken to 
ensure that no genes other than the desired gene are transferred into the 
modified organism and that no undesired proteins are expressed. Drs. Pirone 
and Tblin agreed. 
Dr. Tolin thought the concept of grouping the informational requirements into 
3 broad categories a useful one. Those categories are: (1) a description of 
the material; (2) vectors and methods of introduction; and (3) monitoring. She 
suggested iters one and two of Dr. Vidaver' s document dealt with a description 
of the material; items three through seven dealt with vectors and methods of 
introduction; and the other items with monitoring. 
Dr. Lacy called the working group's attention to iten 5. The working group 
agreed this language was confusing but could not at that time suggest alternate 
language. They suggested, however, several possible situations which should 
be considered: Aqrobacterium , Khizobium , plant viruses, etc. Dr. Pirone said 
the language is too limited in that it uses the word pathogenicity; other 
characteristics might be important considerations. He offered the example of 
ENA coding for enzymes involved in cellulose degradation. He reiterated that 
the working group in developing this document must think in terms of the 
characteristics of the parental organism and the DNA donor. 
Dr. Pirone suggested that a different format, perhaps more like the format of 
the EPA document, might be preferable for the document dealing with microor- 
ganisms. Drs. Lacy and Tolin agreed to attenpt to rewrite the document. 
Dr. Milewski arranged a telephone conference call for 3:30 p.m. for September 21, 
1984. 
Elizabeth Milewski, Ph.D 
Executive Secretary 
[ 46 ] 
