Attachment V - Page 5 
Points to Consider for Submission Under Appendi x 
Release of Mi croorgan 1 sms into the Environment 
Appendix of the Guidelines specifies conditions under 
which certain mi croorgani sms may be approved for release into the 
environment , including field tests. Experiments in this category 
cannot be initiated without submission of relevant information on 
the proposed experiments to N1H for review by the RAC Release of 
Mi croorgani sms Working Group (?) and specific approval by NIH. 
The proposal should include a statement of objectives and a 
description of materials and methods, including methodology for 
monitoring the experiments, and expected results. A summary of 
relevant background and preliminary results should accompany the 
proposal. Information to be submitted should include but not be 
limited to: 
1. Description of mi croorgan i sm ( s ) . Give common and/or 
scientific name of mi croorgan i sm as appropriate. 
2. Give specific strain designations of those you expect to 
use . 
3. Describe the method (s) by which the proposed DNA vector 
will be or has been constructed. Diagrams are very helpful 
and may be necessary for adequate understanding of the 
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