Attachment V - Page 6 
construct. Explain the advantages Cand d i sadvar.tage ( s ) , i f 
appropriate] of you r vectors, if other candidate vectors 
could be considered. 
4. Describe the cloned DMA segment and its expression in the 
new host . 
5. I f mi croorgani sms are used to introduce vectors or are 
vectors themselves, indicate how they compare with 
wild-type strains. I f disabled pathogens are used to 
transmi t the vector or are the vector, indicate factors 
that will most likely prevent these mi croor gan i sms from 
regaining or acquiring pathogenic potential. If the vector 
is likely to survive independently of the host(s), refer to 
this possibility and provide any available data, to assess 
the probability of transfer likely to other organisms. 
6. If a microorganism will only be used to introduce a 
vector (s), document the means by which the presence or 
absence of the microorganism will be assessed. 
7. If the mi croor gan i sm ( s ) or vector (s) are transfer 
deficient, provide some documentation either via the 
proposal or appropriate references. 
8. Provide data from microcosm, greenhouse art d/or growth 
chamber studies under prospective natural conditions. 
[48] 
