Attachment VI - Page 2 
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genetic properties of the host organism would be anticipated as the result 
of any remaining vector ENA. " 
Dr. Vidaver noted that item III-B-3 should be reformated. 
Dr. Pirone suggested the word "likely" be deleted frcm item III-B-3-a-iii. 
Dr. Vidaver suggested the words "pathological, ecological, physiological" be 
substituted for the words "pathologic, ecologic, physiologic" in items III-B-1, 
III-D, and IV-C-3. 
Dr. Vidaver questioned the construction of item D-2. She suggested the language 
should read in part: 
"Provide data related to any anticipated or non- anticipated effects of the 
modified microorganism frcm microcosm, greenhouse, and/or growth chamber 
experiments that s inula te natural conditions. The methods of detection and 
sensitivity of sampling techniques, and periodicity of sampling should be 
indicated. These studies should include assessment of the following items:" 
Dr. Lacy suggested item III-A-4 might more logically be inserted in this document 
as item D-2-d. Dr. Pirone agreed as population considerations would be important 
here. Dr. Lacy felt one consideration under item D-2 would be whether the DNA 
sequences to be introduced into the recipient organism might be adventitiously 
contaminated by other DNA sequences frcm the DNA donor organism. Dr. To Lin 
concerning the homogeneity of the ENA donor organism should be included in the 
document. Dr. Vidaver thought the question of the homogeneity of the donor 
might be addressed in item III-D. 
Dr. Pirone noted that the phrase "including genetics" in items II-B and III-A-2 
is very broad. Dr. Lacy said he had intended the phrase "including genetics" 
to encorpass a request for information on the replication cycle. He noted that 
RAC would need to evaluate as part of its review information on the replication 
cycle of viruses and viroids. Dr. Vidaver suggested the qualifier "relevant" 
be included before the word "genetics" in items II-B and III-A-2. 
Dr. Lacy wondered whether investigators submitting proposals would recognize 
that this request for relevant genetics would apply to RNA as well as DNA. 
Dr. Tolin questioned whether the investigator would include information on 
genetic variability and the stability of gen cmes in their proposals . 
Dr. Lacy agreed that "relevant" might differ for different biological systems 
and agreed to insert this word in items II-B and III-A-2. Dr. Vidaver agreed; 
she felt no document could envisage every possibility. 
Dr. Lacy questioned whether item IV-C-2 should be deleted. Dr. Tolin felt it 
should remain in the document as this section refers to the genetically modified 
organism. 
Dr. Vidaver felt the language of item IV-E was not clear. Dr. Pirone agreed. 
Dr. Lacy said the language of item IV-E was an attempt to request information 
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