Attachment VI - Page 5 
Revisions by G. H. Lacy 
and S. A. Tolin based 
on A. Vidaver’s draft 
of 30 August 1984 
September 18, 1984 
to Consider for Submission Under Section III-A-2. 
Release of Microorganisms Containing Recombinant DNA Molecules into the 
Environment 
Experiments in this category require specific review by the RAC and NIH 
and IBC approval before initiation. Review of experiments under this 
category require submission of relevant information on the proposed 
experiments to ORDA for publication in the Federal Register and inclusion 
on the agenda of a RAC meeting. 
The submission should include a statement of objectives and a description 
of materials and methods, including methodology for monitoring the 
experiments, and expected results. A summary of relevant background 
information and preliminary results conducted under containment should 
accompany the proposal. The following outline has been developed by the 
Working Group for Release into the Environment as a suggested list of 
Points to Consider for scientists preparing proposals. Information 
submitted should include but not be limited to: 
I. Summary of the experiment, including obj-ectives, significance, and 
justification for the request. 
II. Characteristics of the parental microorgani sm to be modified and 
released into the environment 
A. Identification, taxonomy, source, and strain 
B. General biological character i sti cs, including replication cycle 
and genetics. 
C. Pathological, ecological, and physiological traits 
D. Methods for detection and monitoring natural populations of 
this microorganism. Give the sensitivity of these methods. 
III. Characteristics of the modified microorganism to be released into the 
environment 
A. Source of the DNA sequences to be inserted into or used to modify 
the mi croorgani sm to be released into the environment. 
1. Identification, taxonomy, source and strain of organism 
donating the DNA 
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