Attachment VI - Page 7 
2. Provide data related to any anticipated or non-anticipated 
effects of the modified mi croorgani sm from microcosm, 
greenhouse, and/or growth chamber experiments that simulate 
natural conditions. These studies should include assessment 
of the following items. The methods of detection and 
sensitivity of sampling techniques, and periodicity of 
sampling should be indicated. 
a. Survival of the microorgani sm to be released 
b. Replication of the mi croorgani sm to be released 
c. Dissemination routes of the mi croorgani sm 
IV. Release into the environment 
A. Describe the experiment for release of the modified organism into 
the environment. Provide information, including diagrams, of the 
experimental location and the surrounding environs. Information 
provided should include pertinent plant, soil, water, aerobiolo- 
gic, and/or other data to assess the containment or spread of the 
mi croorgani sm. 
B. Indicate containment procedures in the event of accidental re- 
lease as well as intentional release, and procedures for 
emergency termination of the experiment. Specify access and 
security measures for the area(s) in which the tests will be 
perf ormed 
C. If the microorganism to be released is to benefit a plant or 
control another mi croorgani sm, a noxious weed, or an animal pest 
of plants, provide the following information about that target 
organism: 
1. Identification, taxonomy, source, and strain 
2. Genetic characteri sitics 
3. Pathologic, ecologic, and physiologic traits 
4. The anticipated mechanism of the interaction between the 
released microorganism and the target organism 
D. Describe the prospective effects of the introduced microorgani sm 
on non-target plants, animals, and inanimate objects should be 
indicated as appropriate 
E. Describe monitoring procedures and their limits of detection for 
survival, dissemination, and non-target interactions of the modi- 
fied microorganism. Include periodicity of sampling and 
rationales for monitoring procedures. Compare data collected 
with effects of the non-modified wildtype microorgani sm most 
similar to the modified organism at the experimental site. 
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