Attachment VI - Page 9 
September 21, 1984 
POINTS TO CONSIDER FOR SUBMISSIONS INVOLVING RELEASE OF MICROORGANISMS 
CONTAINING RDOOMBIIANT DNA MOLECULES INTO THE ENVIRONMENT 
Experiments in this category require specific review and approval before 
initiation by the Recombinant DNA Advisory Committee (RAC) and the National 
Institutes of Health (NIH) and the Institutional Biosafety Committee (IBC). 
Relevant information on the proposed experiments should be submitted to the 
Office of Recombinant DNA Activities (ORDA). 
The submission should include a statement of objectives and a description of 
materials and methods, including methodology for monitoring the experiments, 
and expected results. A summary of relevant background information and 
preliminary results conducted under containment should accompany the 
proposal. The following outline has been developed by the Working Group 
for Release into the Environment as a suggested list of Points to Consider 
for scientists preparing proposals. Information submitted should include 
but not be limited to: 
I. Summary of the experiment including objectives, significance, and justifica- 
tion for the request. 
II. Character is tics of the parental microorganism to be modified and released 
into the environment. 
A. Identification, taxonomy, source, and strain. 
B. General biological characteristics including replication cycle and 
relevant genetics. 
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