Attachment VI - Page 10 
C. Pathological, ecological, and physiological characteristics. 
D. Methods for detection and monitoring natural populations of this 
microorganism. Describe the sensitivity of these .methods. 
III. Characteristics of the modified microorganism to be released into the 
envirorrnent. 
A. Source of the DMA sequences to be inserted into or used to modify 
the microorganism to be released into the environment. 
1. Identification, taxonomy, source, and strain of organism donating 
the DMA. 
2. General biological characteristics including relevant genetics. 
3. Pathological, ecological, and physiological characteristics. 
B. Genetic manipulations: describe the method(s) by vhich the DMA vector 
has been constructed and introduced. Include diagrams as appropriate. 
1. Describe the nature of the DMA inserted or modified by the genetic 
manipulations. Document the amount of any donor DMA in excess of 
the amount required for the minimal effective genetic alteration in 
the fined, construction. Predict whether any changes in pathological, 
ecological, physiological, or genetic properties of host organism 
would be anticipated as the result of the excess donor DMA. 
2. Describe the amount of any vector DMA remaining in the final 
construction. Predict whether any changes in pathological , 
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