Attachment XI - Page 1 
POINTS TO CONSIDER FOR SUBMISSIONS INVOLVING RELEASE OF MICROORGANISMS 
CONTAINING RECOMBINANT ENA MOLECULES INTO THE ENVIRONMENT 
D | 
! 
Experiments in this category require specific review by the Recanbinant DNA 
Advisory Committee (RAC) and approval by the National Institutes of Health 
(NIH) and the Institutional Biosafety Committee (IBC) before initiation. Relevant 
information on the proposed experiments should be submitted to the Office of 
Recombinant DNA Activities (ORDA). 
The following outline has been developed by the Working Group for Release into 
the Environment as a suggested list of Points to Consider for scientists 
preparing proposals on release into the environment of microorganisms including 
viruses . 
Logical framework for analysis (to be composed later) 
I. SUMMARY OF THE EXPERIMENT INCLUDING OBJECTIVES, SIGNIFICANCE, AND JUSTIFICA- 
TION FOR THE REQUEST 
II. DERIVATION OF RECOMBINANT ORGANISM 
A. Characteristics of the parental microorganism to be modified and released 
into the environment 
1. Identification, taxonomy, source, and strain 
2. Relevant genetics, transfer capability, reproduction cycle(s) 
[ 71 ] 
October 5, 1984 
