Attachment XI ~ Page 2 
2 
B. Molecular Biology of Modifications 
1 . Introduced Genes 
Source of the DNA sequences to be inserted into or used to modify 
the microorganism to be released into the environment 
a. Identification, taxonomy, source, and strain of organism donating 
the DNA. 
b. General biological characteristics including relevant genetics 
2 . Construction of Reccmbinant 
Genetic manipulations: describe the method(s) by which the DNA 
vector has been constructed and introduced. Include diagrams as 
appropriate. 
a. Describe the method of introduction of the DNA and selection of 
altered organisms. 
b. Describe the nature of the DNA inserted or modified by the 
genetic manipulations. Document the amount of any donor DNA in 
excess of the amount required for the minimal effective genetic 
alteration in the final construction. 
c. Describe the amount of any vector DNA remaining in the final 
construction. 
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