Attachment XIV - Page 4 
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a. Describe the method ( s ) by which the vector carrying the insert 
has been constructed. Include diagrams as apprcpriate. 
Describe the nature of the nucleic acid inserted or rrodified 
by the genetic manipulations . 
b. Describe the method of introduction of the vector carrying the 
insert into the modified organisms and selection of altered 
organisms. 
c. Specify the amount and nature of any vector and/or donor DNA 
remaining in the final construction. 
d. Specify the physical and biological containment conditions 
necessary for the modified organism. 
3. Stability and Expression 
a. Stability of recombinant organism. 
b. Expression of introduced genetic material. 
Ill . Environmental Considerations 
The intent of gathering ecological information is to assess the liklihood 
of adverse effects in the event of survival, establishment, or dispersal 
of the modified organism. For this purpose, information should be provided 
where possible and appropriate on: (i) relevant ecological and evolutionary 
characteristics of the nonrrodified (parent) organism; (ii) the corresponding 
characteristics of the modified (recombinant) organism; and (iii) the 
physiological and ecological role of donated genetic sequences in the donor 
organism(s) . 
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