18 
Dr. Gottesnan noted section II-B-2-a requested information on the method by which 
the vector was constructed but did not request information on how the DNA 
insert (s) had been constructed. She suggested section II-B-2-a should read as 
follows : 
"Describe the method (s) by v^ich the vector with insert (s) have been con- 
structed. Include diagrams as appropriate." 
Drs. Vidaver and Pramer suggested section II-B-2-b should read as follows: 
"Describe the method of introduction of the vector carrying the insert into 
the organism to be modified and the procedure for selection of the altered 
organisms . " 
Drs. Vidaver and Sharpies questioned the intent of section II-B-2-d which requested 
the investigator indicate the laboratory containment conditions specified by 
the NIH Guidelines. Dr. Gottesman replied that this requirement was intended 
to elicit information on the containment level specified in the NIH Guidelines 
for use in the laboratory during construction of the organism. 
Dr. Sharpies suggested this section might better read: 
"Give laboratory containment conditions." 
By a vote of eleven in favor, none opposed, and no abstentions, the working 
group accepted this proposed language. 
Dr. Clowes suggested the title of Section II-B-3 should read, Genetic Stability 
and Expression . 
Dr. McGarrity then called the attention of the working group to Section III of 
the document. 
Dr. Vidaver wondered whether the document should state that the organisms to 
be released are intended to fill a beneficial purpose. She thought this concept 
might be included in the second sentence of the preamble of Section III by adding 
the following language: 
"(iv) expected performance of genetic sequences in the modified organism." 
Dr. Pramer suggested the same goal could be achieved by modifying the third 
concept in that sentence to read as follows: 
"(iii) the physiological and ecological role of donated genetic sequences 
in the donor and the modified organism." 
Dr. Gottesman suggested the second paragraph of the prearrtole to Section III might 
read as follows: 
"Approval of small-scale field tests will depend upon the results of labora- 
tory and greenhouse testing of the properties of the modified organism and 
[195] 
