Attachment IV - Page 2 
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Approved of small-scale field tests will depend upon the results of laboratory 
and greenhouse testing of the properties of the modified organism. We antici- 
pate that monitoring of small-scale field tests will provide data on environ- 
mental effects of the modified organism. Such data may be a necessary part of 
the consideration of requests for approval of large-scale tests and commercial 
applications . 
I . Summary 
Present a summary of the proposed trial including objectives, significance, 
and justification for the request. 
II. Genetic Considerations of Modified Organism to be Tested 
A. Characteristics of the Nonmodified Parental Organism 
1. Information on identification, taxonomy, source, and strain. 
2. Information on organism's reproductive cycle and capacity for 
genetic transfer. 
B. Molecular Biology of the Modified Organism 
1. Introduced Genes 
a. Source and function of the DMA sequences used to modify the 
organism to be tested in the environment. 
b. Identification, taxonomy, source, and strain of organism 
donating the DNA. 
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