Attachment IV - Page 3 
3 
2 . Construction of the Modified Organism 
a. Describe the method (s) by which the vector with insert (s) 
has been constructed. Include diagrams as appropriate. 
b. Describe the method of introduction of the vector carrying 
the insert into organism to be modified; describe the procedure 
for selection of organisms. 
c. Specify the amount and nature of any vector and/or donor DNA 
remaining in the modified organism. 
d. Give the laboratory containment conditions specified by the NIH 
Guidelines for the modified organism. 
3 . Genetic Stability and Expression' 
Present results and interpretation of preliminary tests designed to 
measure genetic stability and expression of the introduced DNA in 
the modified organism. 
Ill . Environmental Considerations 
The intent of gathering ecological information is to assess the effects 
of survival, reproduction, and/or dispersed, of the modified organism. 
For this purpose, information should be provided where possible and 
appropriate on: (i) relevant ecological characteristics of the nonmodified 
organism; (ii) the corresponding characteristics of the modified organism; 
and (iii) the physiological and ecological role of donated genetic sequences 
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