Attachment IV - Page 5 
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IV. Trials to be Conducted 
A. Conditions of the Trial 
Describe the tried involving release of the modified organism into the 
environment: 
1. Anounts of organisms and methods of application. 
2. Provide information including diagrams of the experimental location 
arri the immediate surroundings. Describe characteristics of the 
site that would influence containment or dispersal. 
3. If the modified organism has a target organism, provide the 
following: 
a. Identification, taxonomy* and strain; 
b. The anticipated mechanism and result of the interaction between 
the released microorganism and the target organism. 
B. Provide data related to any anticipated or nonant ici pat ed effects of 
the modified microorganism on target and nontarget organisms from 
microcosm, greenhouse, and/or growth chamber experiments that simulate 
trial conditions. The methods of detection and sensitivity of sampling 
techniques and periodicity of sampling should be indicated. These 
studies should include assessment of the following items: 
1. Survival of the modified organism. 
2. Replication of the modified organism. 
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