Attachment IV - Page 6 
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3. Dissemination routes of the modified organism. 
C. Indicate containment procedures in the event of accidental release as 
well as intentional release and procedures for emergency termination 
of the experiment. Specify access and security measures for the area(s) 
in vhich the tests will be performed. 
D. Monitoring 
Describe monitoring procedures and their limits of detection for survival, 
dissemination, and nontarget interactions of the modified microorganism. 
Include periodicity of sampling and rationale for monitoring procedures. 
Collect data to compare the modified organisms with the nonmodi fied 
i microorganism most similar to the modified organism at the site of the 
trial. Results of monitoring should be submitted to RAC. 
V. Risk Assessment 
Summarize risk assessment conclusions and justification for safe conduct of 
experiment . 
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