Federal Register / Vol. 49, No. 227 / Friday, November 23, 1984 / Notices 
46259 
acids, alkalis, organic solvents, and 
moderate heat. 
“Appendix G—ll- B 4 c. Laboratory 
furniture is sturdy and spaces between 
benches, cabinets, and equipment are 
accessible for cleaning. 
"Appendix G-II-B-4-d. Each 
laboratory contains a sink for hand- 
wishing. 
“Appendix G-ll-B- 4 e. If the 
j laboratory has windows that open, they 
are fitted with fly screens. 
"Appendix G-II-B-4-f. An autoclave 
for decontaminating laboratory wastes 
is available.” 
h. The following language is 
! substituted for Appendix G-II-C, P3 
Level. 
“Appendix G-II-C. Biosafety Level 3 
(BL3) [16j. 
"Appendix G-II-C-1. Standard 
Microbiological Practices. 
“Appendix G-II-C-1 -a. Work 
surfaces are decontaminated at least 
once a day and after any spill of viable 
material. 
“Appendix G-II-C-l-b. All 
contaminated liquid or solid wastes are 
decontaminated before disposal. 
| “Appendix G-II-C-l-c. Mechanical 
pipetting devices are used; mouth 
pipetting is prohibited. 
"Appendix G-II-C-1-<1. Eating, 
drinking, smoking, storing food, and 
applying cosmetics are not permitted in 
the work area. 
“Appendix G— II-C-1-e. Persons wash 
their hands after handling materials 
involving organisms containing 
i recombinant DNA molecules, and 
animals, and when they leave the 
laboratory. 
“Appendix G-II-C-l-f. All procedures 
are performed carefully to minimize the 
creation of aerosols. 
“Appendix G-II-C-l-g. Persons under 
18 years of age shall not enter the 
laboratory. 
“Appendix G-II-C-l-h. If experiments 
involving other organisms which require 
lower levels of containment are to be 
conducted in the same laboratory 
concurrently with experiments requiring 
BL3 level physical containment, they 
shall be conducted in accordance with 
all BL3 level laboratory practices. 
"Appendix G-II-C-2. Special 
Practices. 
"Appendix G— U-C-2-a. Laboratory 
doors are kept closed when experiments 
are in progress. 
“Appendix G-U-C-2-b. Contaminated 
materials that are to be decontaminated 
at a site away from the laboratory are 
placed in a durable leakproof container 
which is closed before being removed 
from the laboratory. 
"Appendix G-ll-C-2-c. The 
laboratory director controls access to 
the laboratory and restricts access to 
persons whose presence is required for 
program or support purposes. The 
director has the final responsibility for 
assessing each circumstance and 
determining who may enter or work in 
the laboratory. 
"Appendix G-lI-C-2-d. The 
laboratory director establishes policies 
and procedures whereby only persons 
who have been advised of the potential 
biohazard, who meet any specific entry 
requirements (e.g., immunization), and 
who comply with all entry exist 
procedures enter the laboratory or 
animal rooms. 
"Appendix G-II-C-2— e. When 
organisms containing recombinant DNA 
molecules or experimental animals are 
present in the laboratory or containment 
module, a hazard warning sign 
incorporating the universal biohazard 
symbol is posted on all laboratory and 
animal room access doors. The hazard 
warning sign identifies the agent, lists 
the name and telephone number of the 
laboratory director or other responsible 
pcrson(s), and indicates any special 
requirements for entering the laboratory, 
such as the need for immunizations, 
respirators, or other personal protective 
measures. 
"Appendix G-II-C- 2-f. All activities 
involving organisms containing 
recombinant DNA molecules are 
conducted in biological safety cabinets 
or other physicial containment devices 
within the containment module. No 
work in open vessels is conducted on 
the open bench. 
“Appendix G-H-C-2-g. The work 
surfaces of biological safety cabinets 
and other containment equipment are 
decontaminated when work with 
organism containing recombinant DNA 
molecules is finished. Plastic-backed 
paper toweling used on nonperforated 
work surfaces within biological safety 
cabinets facilitates clean-up. 
"Appendix G-II-C-2— h. An insect and 
rodent control program is in effect 
“Appendix G-II-C-2-i. Laboratory 
clothing that protects street clothing 
(e.g„ solid front or wrap-around gowns, 
scrub suits, coveralls) is worn in the 
laboratory. Laboratory clothing is not 
worn outside the laboratory, and it is 
decontaminated before being laundered. 
"Appendix G-II-C-2-j. Special care is 
taken to avoid skin contamination with 
contaminated materials; gloves should 
be worn when handling infected animals 
and when skin contact with infectious 
materials is unavoidable. 
"Appendix G-Il-C-2-k. Molded 
surgical masks or respirators are worn 
in rooms containing experimental 
animals. 
“Appendix G-II-C-2-1. Animals and 
plants not related to the work being 
conducted are not permitted in the 
laboratory. 
"Appendix G-II-C-2-m. Laboratory 
animals held in a BL3 area shall be 
housed in partial-containment caging 
systems, such as Horsfall units [llj, 
open cages placed in ventilated 
enclosures, solid-wall and -bottom cages 
covered by filter bonnets, or solid-wall 
and -bottom cages placed on holding 
racks equipped with ultraviolet 
irradiation lamps and reflectors. 
Note. — Conventional caging system* may 
be used, provided that all personnel wear 
appropriate personal protective devices. 
These shall include, at a minimum, wrap- 
around gowns, head covers, gloves, shoe 
covers, and respirators. All personnel shall 
shower on exit from areas where these 
devices are required. 
"Appendix G-LI-C-2-n. All wastes 
from laboratories and animal rooms are 
appropriately decontaminated before 
disposaL 
“Appendix G-II-C-2-o. Vacuum lines 
are protected with high efficiency 
particulate air (HEPA) filters and liquid 
disinfectant traps. 
“Appendix G-II-C-2-p. Hypodermic 
needles and syringes are used only for 
parenteral injection and aspiration of 
fluids from laboratory animals and 
diaphragm bottles. Only needle-locking 
syringes or disposable syringe-needle 
units (i.e., needle is integral to the 
syringe) are used for the injection or 
aspiration of fluids containing organisms 
that contain recombinant DNA 
molecules. Extreme caution should be 
used when handling needles and 
syringes to avoid autoinoculation and 
the generation of aerosols during use 
and disposal Needles should not be 
bent, sheared, replaced in the needle 
sheath or guard or removed from the 
syringe following use. The needle and 
syringe should be promptly placed in a 
puncture-resistant container and 
decontaminated, preferably by 
autoclaving, before discard or reuse. 
"Appendix G— II-C-2-q. Spills and 
accidents which result in overt or 
potential exposures to organisms 
containing recombinant DNA molecules 
are immediately reported to the 
laboratory director. Appropriate medical 
evaluation, surveillance, and treatment 
are provided and written records are 
maintained. 
“Appendix G-II-C-2-r. Baseline 
serum samples for all laboratory and 
other at-risk personnel should be 
collected and stored. Additional serum 
specimens may be collected 
periodically, depending on the agents 
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