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Sublist E 
1. One way transfer of Streptococcus mutans 
or Streptococcus lactis DMA into 
Streptococcus sanguis. 
Sublist F 
1. Streptococcus sanguis 
2. Streptococcus pneumoniae 
3. Streptococcus faecalis 
4. Streptococcus pyogenes 
5. Streptococcus mutans 
Appendix B — Classification of 
Microorganisms on the Basis of Hazard 
Appendix B-I, Classification of 
Etiologic Agents. The original reference 
for this classification was the 
publication Classification of Etiological 
Agents on the Basis of Hazard, 4th 
edition. July 1974, U.S. Department of 
Health, Education, and Welfare, Public 
Health Service, Center for Disease 
Control, Office of Biosafety, Atlanta, 
Georgia 30333. For the purposes of these 
Guidelines, this list has been revised by 
the NIH(7). 
Appendix B-I-A Class 1 Agents. All 
bacterial, parasitic, fungal, viral, 
rickettsial, and chlamydial agents not 
included in higher classes. 
Appendix B-I-B. Class 2 Agents. 
Appendix B-I-B-I. Bacteria! Agents. 
Acinetobacter calcoaceticus 
Actinobacillus — all species 
Aeromonas hydrophila 
Arizona hinshawii — all serotypes 
Bacillus anthrccis 
Bordetella — all species 
BorreJia recurrentis, B. vincenti 
Campylobacter fetus 
Campylobacter jejuni 
Chlamydia psittaci 
Chlamydia trachomatis 
Clostridium botu/inum, Cl. chavvoer, Cl. 
haemolyticum. Cl. histolyticirm, Cl noryi. 
Cl. septicum. Cl. tetani 
Corynebacterium diphtheriae. C equi. C 
haemolyticum, C. pseudotuberculosis, C. 
pyogenes, C renale 
Edwardsiella tarda 
Erysipelothrix insidiosa 
Escherichia coli — all enteropathogenic, 
enterotoxigenic, enteroinvasive and strains 
bearing K1 antigen 
Haemophilus ducreyi. H. influenzae 
Klebsiella — ail species and all serotypes 
Legionella pneumophila 
Leptospira interrogans — all serotypes 
Listeria — all species 
Moraxella — all species 
Mycobacteria — all species except those 
listed in Class 3 
Mycoplasma — all species except 
Mycoplasma mycoides and Mycoplasma 
agalactiae, which are in Class 5 
Neisseria gonorrhoeae, N. meningitidis 
Pasteurel/a — all species except those listed in 
Class 3 
Salmonella — all species and all serotypes 
Shigella — all species and all serotypes 
Sphaerophorus necrophorus 
Staphylococcus aureus 
StreptobaciHus moniliformis 
Vol. 49, No. 227 / Friday, November 
Streptococcus pneumoniae 
Streptococcus pyogenes 
Treponema carateum, T. pallidum, and T. 
pertenue 
Vibrio cholerae 
Vibrio parahemolyticus 
Yersinia enterocolitica 
Appendix B-l-B-2. Fungal Agents. 
Aclinomycetes (includiang Nocardia species, 
Actinomyces species, and Arachnia 
propionico)(2 ) 
Blastomyces dermatitidis 
Cryptococcus neo for mans 
Paracoccidioides braziliensis 
Appendix B-I-B-3. Parasitic Agents. 
Endamoeba histolytica 
Leishmania sp. 
Naegleria gruberi 
Schistosoma mansoni 
Toxoplasma gondii 
Toxocara cam's 
Trichinella spiralis 
Trypanosoma cruzi 
Appendix B-I—B—4. Viral, Rjckettsial, 
and Chlamydia 1 Agents. 
Adenoviruses — human — all types 
Cache Valley virus 
Coxsackie A and B viruses 
Cytomegalo viruses 
Echoviruses — all types 
Encephalomyocarditis virus ( EMC] 
Flanders virus 
Hart Park virus 
//epot/t/s -associated antigen material 
Herpes viruses — except Herpesvirus simiae 
[Monkey B virus) which is in Class 4 
Corona viruses 
Influenza viruses — all types except A/PR8/ 
34, which is in Class 1 
Langat virus 
Lymphogranuloma venereum agent 
Measles virus 
Mumps virus 
Parainfluenza virus — all types except 
Parainfluenza virus 3, SF4 strain, which is 
in Class 1 
Polioviruses — all types, wild and attenuated 
Poxviruses — all types except Aiastrim, 
Smallpox, and Whitepox which are Gass 5 
and Monkey pox which depending on 
experiments is in Class 3 or Gass 4 
Rabies virus — all strains except Robies street 
virus which should be classified in Gass 3 
Reo viruses — all types 
Respiratory syncytial virus 
Rhinoviruscs — all types 
Rubella virus 
Simian viruses — all types except Herpesvirus 
simiae (Monkey B virus ) and Marburg 
virus which are in Gass 4 
Sindbis virus 
Tensaw virus 
Turlock virus 
Vaccinia virus 
Varicella virus 
Vesicular stomatitis vinispj 
Vole rickettsia 
Yellow fever virus, 17D vaccine strain 
Appendix B-1-C-. class 3 Agents. 
Appendix B-l-C-1. Bacterial agents. 
Bartone/lo — all species 
Brucella — all species 
23, 1984 / Notices 
Francisella tularensis 
Mycobacterium avium, M. bovis, M. 
tuberculosis 
Pasteurclia muitor.ide type B ("buffalo" and 
other foreign virulent 8trains)(3) 
Pseudomonas mallei(3 ) 
Pseudomonas pseudomallei(3 ) 
Yersinia pestis 
Appendix B-l-C 2. Fungal Agents. 
Coccidioides immitis 
Histoplasma capsulatum 
Histoplasma capsulatum var. duboisii 
Appendix B-l-C-3. Parasitic Agents. 
None. 
Appendix B-l-C— 4. Viral, Rickettsial, 
and Chlamydia Agents. 
Monkey pox, when used in vitro(4) 
Arboviruses — all strains except those in 
Class 2 and 4 ( Arboviruses indigenous to 
the United States are in Class 3 except 
those listed in Class 2. West Nile and 
Semliki Forest viruses may be classified up 
or down depending on the conditions of use 
and geographical location of the 
laboratory.) 
Dengue virus, when used for transmission or 
animal inoculation experiments 
Lymphocytic choriomeningitis virus (LCM) 
Rabies street virus 
Rickettsia — all species except Vole rickettsia 
when used for transmission nor animal 
inoculation experiments 
Yellow fever virus — wild, when used in vitro 
Appendix B-1-D-. Class 4 Agents. 
Appendix B-T-D-l. Bacterial Agents. 
None. 
Appendix B-I-D-2. Fungal Agents. 
Nona. 
Appendix B-l-D-3. Parasitic Agents. 
None. 
Appendix B-I-D— 4. Viral, Rickettsial, 
and Chlamydial Agents. 
Ebola fever virus 
Monkey pox, when used for tramsmfssion or 
animal inoculation experiments^) 
Hemorrhagic, fever agents, Including Crimean 
hemorrhagic fever, (Congo). Junin, and 
Machupo viruses, and others as yet 
undefined 
Herpesvirus simiae (Monkey B v/'rus) 
Lassa virus 
Markburg virus 
Tick-borne encephalitis virus complex, 
including Russian spring-summer 
encephalitis, Kyasanur forest disease, 
Omsk hemorrhagic fever, and Central 
European encephalitis viruses 
Venezuelan equine encephalitis virus, 
epidemic strains, when used for 
transmission or animal inoculation 
experiments 
Yellow fever virus — wild, when used for 
transmission or animal Inoculation 
experimetns 
Appendix B-I I. Classification of 
Oncogenic Viruses on the Basis of 
Potential Hazard (5). 
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