Attachment IV - Page 1 
RECOMBINANT DNA ADVISORY COMMITTEE 
PI Growth Conditions for Plants 
If the IBC requires PI growth conditions for 
plants, this can be met by either (1) a limited 
access greenhouse, or (2) a plant growth 
chamber which are insect-restrictive and in 
which a pest control regime is maintained. 
Sterilization of run-off water is required only 
where this is a plausible route for dissemina- 
tion of viable microorganisms containing 
recombinant DNA. Soil, plant parts, and 
unwanted plant material shall be sterilized be- 
fore disposal. Plant materials which have to 
be removed from the greenhouse or cabinet 
for further research shall be maintained under 
good laboratory practices as applied to 
plants. Plants can be transported locally, e.g., 
between laboratory, growth chamber, and 
greenhouse, at physically separated locations 
provided: (1) plants are in a vegetative condi- 
tion, i.e., no reproductive organs or structures 
(e.g., pollen, flowers, seeds) are present or 
the reproductive organs of the plants are cov- 
ered to prevent the dispersal of reproductive 
cells and spores: (2) plants are kept in an en- 
closed area or container (i.e., waterproof, 
insect-restrictive); and (3) plants are essen- 
tially ‘hand-couriered’ between locations. 
February 6, 1984 
[319] 
