Attachment V - Page 5 
5 
TV. Proposed Field Trials 
A. Pre-Field Trial Considerations 
Provide data related to any anticipated effects of the modified 
microorganism on target and non target organisms frcm microcosm, 
greenhouse, and/or grcv/th chamber experiments that simulate 
trial conditions. The methods of detection and sensitivity of 
sampling techniques and periodicity of sampling should be indicated. 
These studies should include, where relevant, assessment of the 
following items: 
1. Survival of the modified organism. 
2. Replication of the modified organism. 
3. Dissemination of the modified organism by wind, v^iter, soil, 
mobile organisms, and other means. 
B. Conditions of the Trial 
Describe the trial involving release of the modified organism into the 
environment : 
1. Numbers of organisms and methods of application. 
2. Provide information including diagrams of the experimental location 
and the immediate surroundings. Describe characteristics of the 
site that would influence containment or dispersal . 
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