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Attachment V - Page 7 
the information requested in Sections II, III, and IV should be summarized 
to present an analysis of possible risks to the environment in the test as it 
is proposed. The issues addressed might include but not be limited to the 
follcwino items: 
A. The Nature of the Organism 
1. The role of the nomodi fied organism in the environment of the 
test site, including any adverse effects on other organisms. 
2. Evaluation of vhether or not the specific genetic modification 
(e.g., deletion, insertion, nodification of specific DNA sequences) 
would alter the potential for significant adverse effects. 
3. Evaluation of results of tests conducted in contained environments 
to predict the ecological behavior of the modified organism relative 
to that of its no modi fied parent. 
R. The Nature of the Test 
Discuss the following specific features of the experiment that are 
desiqned to minimize potential adverse effects of the modified organism: 
1. Test site location and area. 
2 . Introduction protocols . 
3. Numbers of organisms and their expected reproductive capacity. 
4. emergency procedures for aborting the experiment. 
5. Procedures conducted at the termination of the experiment. 
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