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Federal Register / Vol. 51, No. 88 / Wednesday, May 7, 1980 / Notices 
Vole rickettsia 
Yellow fever virus, 17D vaccine strain 
Appendix B-I-C. Class 3 Agents. 
Appendix B-I-C-l. Bacterial Agents. 
Bartonella — all species 
Brucella — all species 
Francisella tularensis 
Mycobacterium avium, M. bovis, M. 
tuberculosis 
Pasteurella multocide type B ("buffalo" 
ar.d other foreign virulent strains) [3] 
Pseudomonas mallei [3] 
Pseudomonas pseudomallei [3) 
Yersinia pestis 
Appendix B-I-C-2. Fungal Agents. 
Coccidioides immitis 
Histoplcsma capsulatum 
Histoplasrr.a capsulatum var. duboisii 
A.ppendix B-I-C-3. Parasitic Agents. 
None. 
Appendix B-l-C-t. Viral, Rickettsial, 
and Chlamydial Agents. 
Monkey pox. when used in vitro [4] 
Arboviruses- all strains except these in 
Class 2 and 4 [Arboviruses indigenous to 
the United States are in Class 3 except 
those listed in Class 2. West Nile and 
Semliki Fcrest viruses may be classified 
up or down depending on the conditions 
of use and geographical location of the 
laboratory.) 
Dengue virus, when used for transmfssion 
or animal inoculation experiments 
Lymphocytic choriomeningitis virjs (LCM) 
Rickettsia — all species except Vole 
rickettsia when used for transmission or 
animal inoculation experiments 
Yellow fever virus — wild, when used in 
vitro 
Appendix B-l-D. Class 4 Agents. 
Appendix B-I-D-l. Bcctericl Agents. 
None. 
Appendix B-I-D-2. Fungal Agents. 
None. 
Appendix B-I-D-3. Parasitic Agents. 
No ns. 
Appendix B-I-D-4. Viral. Rickettsial, 
and Chlamydial Agents. 
Ebola fever virus 
Monkey pox. when used for transmission 
or animal inoculation experiments [4] 
Hemorrhagic fever agents, including 
Crimean hemorrhagic fever. (Congo), 
funin. and Machupo viruses, and others 
as yet undefined 
Herpesvirus simiae (Monkey B virus ) 
Lassa virus 
Marburg virus 
Tick-borne encephalitis virus complex. 
including Russian spring-summer 
encephalitis. Kyasanur forest disease. 
Omsk hemorrhagic fever, and Central 
European encephalitis viruses 
Venezuelan equine encephalitis virus. 
epidemic strains, when used for 
transmission or animal inoculation 
experiments 
Yellow fever virus — wild, when used for 
transmission or animal inoculation 
experiments 
Appendix B-1I — Classification of 
Oncogenic Viruses on the Basis of 
Potential Hazard [5] 
Appendix B-II-A. Low-Risk 
Oncogenic Viruses. 
Rous sarcoma 
SV— 40 
CELO 
Ad7-SV40 
Polyoma 
Bovine papilloma 
Rat mammary tumor 
Avian leukosis 
Murine lpuksmia 
Murine sarcoma 
Mouse mammary tumor 
Rat leukemia 
Hamster leukemia 
Bovine leukemia 
Dog sarcoma 
Mason-Pfizer monkey virus 
Marek's 
Guinea pig herpes 
Lucke (Frog) 
Adenovirus 
Shope fibroma 
Shope papilloma 
A.ppendix B-1I-B. Moderate-Risk 
Oncogenic Viruses. 
Ad2-SV40 
FeLV 
HV Saimiri 
EEV 
SSY-1 
GaLV 
HV ateles 
Yaba 
FeSV 
Appendix 3-111 — Class 5 Agents 
Appendix B-III-A. Animal Disease 
Organisms Which are Forbidden Entry 
into the United States by Law. 
Foot and mouth disease virus. 
Appendix B-III-B. Animal Disease 
Organisms and Vectors Which are 
Forbidden Entry into the United States 
by USD A Policy. 
African horse sickness virus 
African swine fever virus 
Besnoitia bssnoiti 
Boma disease virus 
Bovine infectious petechial fever 
Camel pox virus 
-Ephemeral fever virus 
Fowl plague virus 
Goat pcx virus 
Hog cholera virus 
Louping ill virus 
Lumpy skin disease virus 
Nairobi sheep disease virus 
Newcastle disease virus (Asiatic strains) 
Mycoplasma mycoides (contagious bovine 
pleuropneumonia) 
Mycoplasma agalactiae (contagious 
agalactia of sheep) 
Rickettsia ruminatium (heart water) 
Rift valley fever virus 
Rhinderpest virus 
Sheep pox virus 
Swine vesicular disease virus 
Teschen disease virus 
Trypanosoma vivex (Nagana) 
Trypanosoma evansi 
Theileria pan'a (East Coast fever) 
Theileria annulata 
Theileria lawrencei 
Theileria bovis 
Theileria hirci 
Vesicular exanthema virus 
Wesselsbron disease virus 
Zyonerna 
Appendix B-III-C. Organisms Which 
may not be Studied in the United States 
Except at Specified Facilities. 
Small pox [4] 
Alastrim [4] 
White pox [4] 
Appendix B-IV— Footnotes and 
References of Appendix B. 
1. The original reference for this 
classification was the publication 
Classification of Etiologic Agents on the 
Basis of Hazard, 4th edition. July 1974, U.S. 
Department of Health. Education, and 
Welfare, Public Health Service, Center for 
Disease Control. Office of Biosafety, Atlanta, 
Georgia 30333. For the purposes of these 
Guidelines, this list has been revised by the 
NIH. 
2. Since the publication of the classification 
in 1974 [1], the Actinomycetes have been 
reclassified as bacterial rather than fungal 
agents. 
3. A USDA permit, required for import and 
interstate transport of pathogens, may be 
obtained from the Animal and Plant Health 
Inspection Service, USDA, Federal Building, 
Hyattsville. MD 207e2. 
4. All activities, including storage of variola 
and whitepox, are restricted to the single 
national facility [World Health Organization 
(WHO) Collaborating Center for Smallpox 
Research, Centers for Disease Control, in 
Atlanta]. 
5. National Cancer Institute Scfety 
Standards for Research Involving Oncogenic 
Viruses (October 1974). U.S. Department of 
Health, Education, and Welfare Publication 
No. (NIH) 75-790. 
6. U.S. Department of Agriculture, Animal 
and Plant Health Inspection Service. 
Appendix C — Exemptions Under 
Section III-D-5 
Section III-D-5 states that exempt 
from these Guidelines are “Other 
classes of recombinant DNA molecules 
if the Director, NIH, with advice of the 
RAC, after appropriate notice and 
opportunity for public comment finds 
that they do not present a significant 
risk to health or the environment (see 
Section IV-C-l-b-(l}-(c)). Certain 
classes are exempt as of publication of 
these revised Guidelines.” 
The following classes of experiments 
are exempt under Section III-D-5 of the 
Guidelines: 
[12] 
Recombinant DNA Research, Volume 1 1 
