Federal Register / Vol. 51,. No. 88 / Wednesday, May 7, 1986 / Notices 
16967 
DNA by known physiological processes 
though one or more of the segments may 
be a synthetic equivalent. A list of such 
exchangers will be prepared and 
periodically revised by the Director. 
NIH. with advice of the RAC after 
appropriate notice and opportunity for 
public comment (see Section IV-C-l-b- 
(iHc))- Certain classes are exempt as of 
publication of these revised Guidelines. 
The list is in Appendix A." 
Under Section III-D-1 of these 
Guidelines are recombinant DNA 
molecules that are: (1) Composed 
entirely of DNA segments from one or 
more of the organisms within a sublist 
and (2) to be propagated in any of the 
organisms within a sublist. 
(Classification of Bergey's Manual of 
Determinative Bacteriology. 8th edition. 
R. E. Buchanan and N. E. Gibbons, 
editors. Williams and W'ilkins Company: 
Baltimore. 1974.) 
Although these experiments are 
exempt, it is recommended that they be 
performed at the appropriate biosafety 
level for the host or recombinant 
organism (for biosafety levels see 
Biosafety in Microbiological and 
Biomedical Laboratories. 1st Edition 
(March 1984). U.S. Department of Health 
and Human Services. Public Health 
Service, Centers for Disease Control 
Atlanta. Georgia 30333. and National 
Institutes of Health, pethesda. Maryland 
20892). 
Sublist -4 
1. Genus Escherichia 
2. Genus Shigella 
3. Genus Sclmonella (including Arizona) 
4 . Genus Enterobac'.er 
5. Cenus Citrobacter (including Levinea) 
8. Cenus Klebsiella 
7. Genus Er.vinia 
8. Pseudomonas aeruginosa. Pseudomonas 
Pulida and Pseudomonas fluorescens 
9. Serratia morcescens 
10. Yersinia enterocolitica 
Sublist B 
1. Bacillus subtilis 
2. Bacillus licheniformis 
3. Bacillus pumilus 
4 . Bacillus globigii 
5. Bacillus niger 
8. Bacillus nato 
7. Bacillus amyloliquefaciens 
8. Bacillus aterrimus 
Sublist C 
1. Streptomycss aureofaciens 
2. Streptomyces rimosus 
3. Streptomyces coelicolor 
Snblist D 
1. Streptomyces griseus 
2. Streptomyces cyar.eus 
3. Streptomyces venezuelae 
Recombinant DNA Research, 
Sublist E 
1. One way transfer of Streptococcus 
mutans or Streptococcus lactis DNA into 
Streptococcus sanguis. 
Sublist F 
1. Streptococcus sanguis 
2. Streptococcus pneumoniae 
3. Streptococcus faecalis 
4 . Streptococcus pyogenes 
5. Streptococcus mutans 
APPENDIX B — CLASIFICATION OF 
MICROORGANISMS ON THE BASIS 
OF HAZARD 
Appendix B-I — Classification of 
Etio logic Agents 
The original reference for this 
classification was the publication 
Classification of Etiological Agents on 
the Basis of Hazard. 4th edition. July 
1974, U.S. Department of Health. 
Education, and Welfare. Public Heslth 
Service. Center for Disease Control. 
Office of Biosafety. Atlanta. Georgia 
30333. For the purposes of these 
Guidelines, this list has been revised by 
the NIH (1). 
Appendix B-I-A. Class 1 Agents. All 
bacterial, parasitic, fungal, viral, 
rickettsial, and chlamydial agents not 
included in higher classes. 
Appendix B-l-B. Class 2 Agents. 
Appendix B-l-B-1. Bacteria! Agents. 
Acinetobacter calcoaceticus 
Actinobacillus-a)) species 
Aeromonas hydrophila 
Arizona hinshawii-aW serotypes 
Bacillus anthracis 
Bordetella - all species 
Borrelia recurrentis. B. vincenti 
Campylobacter fetus 
Campylobacter jejdni 
Chlamydia psittaci 
Chlamydia trachomatis 
Clostridium botulinum. 
Cl. chauvoei. CL haemolyticum. 
Cl. histolyticum. Cl. novyi. 
Cl. septicum. Cl. letani 
Corynebacterium diphtheriae. 
C. equi, C. haemolyticum. 
C. pseudotuberculosis. 
C. pyogenes. C. renale 
Edwardsiella tarda 
Erysipelothrix insidioso 
Escherichia coli - all enteropathogenic. 
enterotoxigenic, enteroir.vasive and 
strains bearing Kl antigen 
Haemophilus ducreyi. H. influenzce 
Klebsiellas.)) species and all serotypes 
Legionella pneumophila 
Leptospira interrogans-a)) serotypes 
Listeria - all species 
Moraxella-a)) species 
Mycobacteria - all species except those 
listed in Class 3 
Mycoplasma-a)) species except 
Mycoplasma mycoides and Mycoplasma 
agolactioe. which are in Class 5 
Neisseria gonorrhoeae. N. meningitidis 
Posteurella - all species except those listed 
in Class 3 
Salmonello-a)) species and all serotypes 
Volume 1 1 
Shigella-a)) species and all serotypes 
Sphcerophorus necrophorus 
Staphylococcus aureus 
Slreptobacillus moniliformis 
Streptococcus pneumoniae 
Streptococcus pyogenes 
Treponema carateum. T. pallidum, end T. 
pertenue 
Vibrio cholerae 
Vibrio parahemolyticus 
Yersinia enterocolitica 
Appendix B-I-B-2. Fungal Agents. 
Actinomycetes (including Nocardio 
species. Actinomyces species, and 
Arachnia prcpionica) (2) 
Blastomyces dermatitidis 
Cryptococcus neoformans 
Paracoccidioides brazihensis 
Appendix B-I-B-3. Parcsitic Agents. 
Entlamoeba histolytica 
Leishmania sp. 
Kaegleria gruberi 
Schistosoma mansoni 
Toxoplasma gondii 
Toxocara cams 
Trichine/la spiralis 
Trypanosoma cruzi 
Appendix B-l-B-1. Viral. Rickettsial, 
and Chlamydia! Agents. 
Adenoviruses — human — all types 
Cache Valley virus 
Coxsackie A end B viruses 
Cytomegaloviruses 
Echoviruses — all types 
Encephalomyocarditis virus (EMC) 
Flanders virus 
Hart Park virus 
Hepatitus — associated antigen material 
Herpes viruses-ex cept Herpesvirus simice 
(Monkey B virus) which is in Class 4 
Corona viruses 
Influenza viruses — all types except A/PR8 / 
34. which is in Class 1 
Langat virus 
Lymphogranuloma venereum agent 
Measles virus 
Mumps virus 
Parainfluenza virus — all types except 
Parainfluenza virus 3, SF4 strain, which 
is in Class 1 
Polioviruses — all types, wild and 
attenuated 
Poxviruses — all types except Alostrim. 
Smallpox, and Whitepox which are Class 
5 and Monkey pox which depending on 
experiments is in Class 3 or Class 4 
Babies virus — all strains except Rabies 
street virus which should be classified in 
Class 3 
Reoviruses — all types 
Respiratory syncytia / virus 
Rhinoviruses — all types 
Rubella virus 
Simian viruses — all types except 
Herpesvirus simiae (Monkey B virus) 
and Marburg virus which are in Class 4 
Sindbis virus 
Tensaiv virus 
Turlock virus 
Vaccinia virus 
Varicella virus 
Vesicular stomatitis virus [3| 
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