Therefore, the working group presents the following two options for 
public comment and RAC consideration: 
"1. Change definition of recanbinant DNA: 
"The first paragraph of Section I-B would be revised to read as follows 
(new words underlined): 
"In the context of these Guidelines, recanbinant ENA molecules 
are defined as either: (i) molecules which are constructed 
outside living cells by joining foreign natural or foreign 
synthetic DNA segments to DNA molecules that can replicate in 
a living cell, or (ii) DNA molecules that result fran the 
replication of those described in (i) above. 
"The following new footnote would be added at the word 'foreign': 
"Rearrangements involving the introduction of DNA frcm different 
organisms or different strains of an organism will be considered 
recombinant DNA. Deletions, single-base changes and rearrangements 
within a single gencme will not involve the introduction of foreign 
DNA and therefore would not be considered recanbinant DNA." 
"2. Modify Section III-A-2 to read as follows: 
"III-A-2. Deliberate release into the environment of any organisn 
containing recanbinant ENA except those listed below. The term 'deliberate 
release' is defined as a planned introduction of recanbinant DNA-containing 
microorgan ims, plants, or animals into the environment. 
"a. Introductions conducted under conditions considered to be accepted 
scientific practices in which there is adequate evidence of biological 
and/or physical control of the recombinant DNA-containing organisms. 
The nature of such evidence is described in Appendices L, M, N, and 0. 
"b. Deletion derivatives and single base changes not otherwise 
covered by the Guidelines. 
"c. Rearrangements and amplification within a single gencme. Rearrange- 
ments involving the introduction of DNA frcm different strains of 
the same organism would not be covered by this exemption." 
After voting 9 in favor, 1 opposed, and no absentions on the first sentence 
of the motion, the working group voted 9 in favor, none opposed, and 1 
[146] 
Recombinant DNA Research, Volume 1 1 
