Construction of Specific Gene Deletion 
Chromosomal of 
original organism 
\j xl yjJ a** i-t-J 
/ 
-<=Z===Z=ZJ 
~~ r . - 
V 
5 
\ 
Excised segment, prepared by treating with a restrictive enzyme 
which raxes cuts at specific locations.' ^ - X) 
The excised gene with sane extra DiA at each end is then ligated to DNA 
of a vector plasnid that has been treated with the same restriction enzyme. 
The hybrid vector plasmid is transferred to E. coli bacteria to 
allow multiplication of cloned gene. 
[248] 
Recombinant DNA Research, Volume 11 
