Homologous DNA regions line up; recombination between plasmid and 
chromosome occurs at x's replacing intact gene with deletion-containing 
derivative; plasmid, which new contains intact gene again, cannot 
survive in this organism and is lost. 
Net effect is the precise removal of a segment of the native gene, leaving 
behind no foreign DNA and resulting in no other change in the organism's genetic 
material. 
Note that the biology and biochemistry is such that each step in the 
outline has a very low probability of occurrence. Splicing technology is 
totally dependent on the pewer of microbial genetics, which enables one to 
select for these rare events. 
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