NOTICES 
33079 
Table III 
Reocrmended Containment for Cloning of Viral Dtft or cDtR in E. c ol i K-12 Host-Vector Systems 
(See text for full details) 
Type of viral ENA secynent to be cloned 
Virus class 
Si±>genanic[38] 
Gencmic 
cOtR from 
viral mRNA[371 
ton transforming 
segment 
Segment contain- 
ing an entire 
transforming qene 
Non-segrrented 
genome 
Segmented 
gencme 
cm 
Nontransforming viruses 
AAV, MW, Mouse Adeno (Strain FL) 
PI + EKl 
PI + EKl 
Pi + EKl 
Plant Viruses 
PI + EKl 
PI + EKl 
Pi + EKl 
Hepatitis B 
PI + EKl 
P2 + EK2 
P2 ♦ EK1CV(401 
or P3 + EX1 
or P3 + EKl 
Other 
PI + EKl 
Pi + EK1CV140) 
PI + EKl 
Transforming Viruses 
Herpes Saimiri, H. Ateles and 
PI + EX1 
P2 + EK1CV[40) 
P2 + EK2 
P2 + EK1CV(40) 
EBV[39] 
or P3 + EK1 
or P3 + EKl 
or P3 + EKl 
Other 
PI + EX1 
P2 + EK1CV[40) 
P2 + EK1CV[40] 
P2 + EK1CV(40] 
or P3 + EK1 
or P3 + EKl 
or P3 + EKl 
vm 
Retrcvi ruses 
Gibbon Ape, Woolly Monkey 
PI + EX1 
P2 + EK1CV[40J 
P2 + EK2 
P2 + EK2 
FeLV and FeSV[39) 
or P3 + EX1 
or P3 + EKl 
or P3 «- EKl 
Other 
PI + EKl 
P2 + EKJ.CV [40] 
P2 + EK1CV[40] 
P2 + EK1CV140) 
or P3 + EKl 
or P3 + EKl 
or P3 + EKl 
•raoie ill 
Recommended Containment for Cloning of Viral OR or cWR in E. coli K-12 Host-Vector Systems 
(See text for full details) 
Type of viral DNA segment to be cloned 
Virus class 
Subgencmic[38] 
Genomic 
cDtR from 
viral imRNA[37] 
Iton transforming 
segment 
Segment contain- 
ing an entire 
transforming gene 
Nonsegment el 
. genome 
Segmented 
genome 
Negative Strand RFR 
PI + EKl 
PI + EKl 
PI + EXl 
PI + EKl 
Plus Strand RtR 
Types 1 and 2 Saoin Polio, 17D 
Yellow Fever Vaccine Strains 
PI + EKl 
PI + EKl 
PI + EKl 
Other 
PI + EKl 
P2 + EK1CV140] 
or P3 + EKl 
P2 ♦ EK1CV(40] 
or P3 + EKl 
Do role Stranded RNA 
PI + EKl 
PI + EKl 
Pi ♦ EKl 
Plant Viruses ♦ Viroids 
PI + EKl 
PI + EKl 
PI +■ EKl 
PI + EKl 
Intracellular Viral DNA 
See text 
See text 
See text 
FEDERAL REGISTER, VOL. 43, NO. 146— FRIDAY, JULY 28, 1978 
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