33126 
NOTICES 
oqhpariscn or hie awruwir levels of nit 1976 ojilklines 
Aiffi HIE I RjKStll REVISED GUIDELINES 
INSERT!!) U* 
CUroil WIOfT LEVELS 
JUNE 1974 GUIDELDCS 
RAC PHOTOS Hi 
REVISED GUIDELINES 
NIH DIRECTOR'S 
REVISED GUIDELINES 
niYSICAL biological 
RIYSICAL BIOLOGICAL 
PHYSICAL BIOLOGICAL 
44. Plukaryut lc pilaaxid and 
phage which rkj not exchange 
information with K. coll 
Sane as shntgin 
conditions for hast 
Same as shot gin 
conditions for hest 
Same as shotgin 
conditions for host 
49. alnimue ooitaiiment 
PI EK1 
or P2 EK2 
— — 
— — 
50. Characterized Clones 
See Characterized clones 
(Category 27-28) 
1BC may approve single- 
step reduction in physical 
or biological containment 
I DC may approve sirrjle- 
step reduction in physical 
or biological containment, 
except for prisiate DNA 
Reduction of sore than one 
step requires NIH approval 
Reduction of more than coo 
step, or reduction of pri- 
mate OtlA requires NIH 
approval . 
Animal Itost-Vertor Systems 
Defective polyoma virus 
51. Containing l» of non- 
imthogrnic organise 
52. Containing class 2 anueal 
vi rim tefiencn 
PI cul tired cells 
P4 cultired cells 
See Categories 53-60 
Sec Categories 53-60 
• ft 
• ft 
51. wtien clone shown to contain 
only lunlm regions 
rl oil tired cells See Categories 58-60 
refect tve SV40 virus 
54. 
I»h froe any non- pathogenic 
organ m or Class 1 vine 
P4 
established cell 
lines 
See Categories 61-65 
• ft 
55. 
If Inserted segment is 
purified prokaryotic t»4A or 
previously cloned eiAaryotic 
INA Unra finction is knevn 
PI 
est^il lsled cell 
lines 
See Categories 61-65 
• ft 
56. 
If defective SV40 lacks 
late reipion, there is no 
helper virus, and no 
infectious virus is being 
pxcxiuced 
PI 
establisixxl cell 
lines 
See Categories 61-65 
• ft 
57. 
if nm- permissive cells 
trams fotmed 
PI 
establ isliod nor 
permissive cell 
lines 
See Categories 61-65 
• ft 
Defective or Intact polyomi 
vine 
53. 
Containing DNA of Class 1 or 


n cultired cells 
• ft 
Class 2 bacteria, except for 
species producing potent 
pnlypept l.te toxins 
HDOLAl Mount. VOC 43, NO. 146— F9IDAY, JULY 33, 1973 
[ 97 ] 
