NOTICES 
33137 
OOMP ARISON OF HIE OCNIAKWEVT LEVELS OF HIE 1976 GUIDELINES 
AND HIE PICPOSED REVISED GUIDELINES 
1NSKR1TD UNA 
CJUtflAINiaJT LEVELS 
RAC PROPOSED NIII DIRECTOR’S 
JUNE 1976 GUIDELINES REVISED GUIDELINES REVISED GUIDELINES 
PHYSICAL BIOLOGICAL PIIYSICAI. BIOLOGICAL BIYSICAL BIOLOGICAL 
59. Containing DMA of eukaryote — — P3 cultured cells 
not producing potent poly- 
(eptide toxin 
Un conditionally defective polycma 
virtE 
60. Sitvgenunic segments of Class 1 — — PI cultured cells 
or Class 2 viruses vrfiich do not 
leplicate in mouse cells 
SV40 ami Adenovirus Types 2 
and S 
Unoxvlitional ly defective by 
deletion in essential gene 
reg ion 
61. if insertol DNA is fran — — M cultured cells 
Class 1 or 2 bacteria (except 
for species producing potent 
polypei* irle toxins) or fran 
eukaryote not producing 
potent polypeptide toxin 
62. if inserted DNA is purified — — P3 cultured cells 
prokaryotic segment, or 
identified segment of 
eukaryotic DNA previously 
clone.! in prokaryotic 
I «;t- vector system 
63. Unconditionally defective 
by deletion in capsid genes 
64. 
containing DNA fran Class 1 
agents 
P4 
established 
cell line 
• • 
if vector is known lo be 
free of complete viral 
genano 
P3 
established 
cell line 
65. 
SV40 or Ad. 2 and 5 DNA plus 
sitogenomic sequences fran 
— 
— 
P3 
non- permissive 
cells 
ft* 
eikaryotic organisms or 
Class 1 or 2 agents; reca tv- 
fa inant molecule defective and 
no virus being produced 
FEDERAL REGISTER, VOL 43, NO. 146— FRIDAY, JULY 2t, 1973 
[ 98 ] 
