5. Residual Action of Disinfectants 95 
6. Laboratory Spills 96 
7. Disposal 100 
8. Selecting Chemical Disinfectants in 
Recombinant DNA Research 102 
9. Caution Required When Applying Disinfectant 
Methods 106 
F. Care and Use of Laboratory Animals 108 
1. Care and Handling of Infected Animals 109 
2. General Guidelines that Apply to Animal 
Room Maintenance Ill 
3. Necropsy Rules for Infected Animals 112 
G. Biohazard Symbol and Its Use 113 
H. Protection of Vacuum Systems When Filtering 
Biohazardous Materials 117 
III. Containment Equipment 121 
A. The Biological Safety Cabinet 121 
1. Class I Biological Safety Cabinet 123 
2. Class II Biological Safety Cabinet 125 
3. Class III Biological Safety Cabinet 128 
B. Certification Procedures 130 
1. Certification of the Face Velocity of 
the Class I Cabinet 132 
2. Certification of the Face Velocity of 
the Class II, Type A Cabinet 133 
3. Certification of the Face Velocity of 
the Class II, Type B Cabinet 134 
4. Certification of the Velocity Profile of 
the Class II Cabinet 135 
5. Certification of the Leak Tightness of 
the Class II, Type A Cabinet 136 
6. Certification of Leak Tightness of the 
Class III Cabinet Systems 138 
7. Leak Testing of High-Efficiency Particulate 
Air Filters (HEPA) 142 
8. Certification of the Operational Negative 
Air Pressure in the Class III Biological 
Safety Cabinet System 145 
IV. Special Laboratory Design 147 
A. The P3 Facility 148 
B. The P4 Facility 151 
C. Certification Procedures 156 
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