1. Certification of Leak Tightness of 
Penetration Seals for P4 Suit Rooms 
Without Primary Barriers 158 
2. Certification of Directional Airflow 160 
3. Certification of Negative Pressure 
Within P4 Suit Areas 161 
4. Certification of the Leak Tightness of 
Exhaust Air Ducts from the P4 Suit Area 162 
5. Certification of Leak Tightness of 
General Exhaust Air Ducts (New Construction) . . 164 
6. Certification of Steam and Ethylene Oxide 
Sterilizer* 167 
7. Leak Testing of High-Efficiency 
Particulate Air (HEPA) Filters 170 
8. Certification of the P4 Facility Liquid 
Biowaste Treatment System 171 
V. Packaging and Shipping of Recombinant DNA Materials ... 173 
VI. Roles and Responsibilities 181 
A. The Institutional Biosafety Committee 181 
B. The Biological Safety Officer 191 
C. Emergency Procedures 194 
D. Medical Surveillance 196 
E. Training Aids, Materials and Courses 206 
F. Reference Bibliography on Biological Safety 210 
VII. NCI Guidelines for Control of Moderate Risk 
Oncogenic Viruses 222 
