Guidelines should reflect differences in the virulence of individual 
plant pathogenic bacteria with a general level of P2 + EK2 for cloning 
in E. coli K-12. She stated that to her knowledge the only plant 
pathogen bacterial DNA currently being cloned is the tumor inducing 
DNA of Ag robacterium tumifaciens vhich is now at P2 + EK2 but in the 
revised Guidelines would be either at P3 + EK2 or not restricted 
at all. A final point was made by Leslie Dach (Environmental Defense 
Fund) who asked for a better rationale in the Experimental section 
for the categorization of certain classes of experiments into specific 
physical and biological levels. 
R oles and Responsibilit ies 
Dr. Fredrickson introduced this section by noting that the 
Guidelines refer to NIH grantees but are also subscribed to by the 
NSF and USDA. He believed that the Guidelines will continue to 
evolve as more groups came under their aegis. Dr. Fredrickson then 
asked Dr. William Gar tl and (ORDA) to provide a chronology of events 
for the implementation of the Guidelines. Dr. Gartland indicated 
that prior to promulgation of the Guidelines in June 1976, NIH sent 
letters of information to its grantees which stated that guidelines 
were under consideration and asked each principal investigator to 
return a statement of agreement to abide by the Asilomar guidelines. 
Following publication of the NIH Guidelines, ORDA sent notices to 
both institutions and principal investigators establishing Drocedures 
xi 
[ 197 ] 
