5 
Appendix D as a safety manual for local institutions where recombinant 
DNA research is taking place. Additionally, in view of the large 
number of recommendations for training courses in safety, the NIH 
has awarded a contract to the American Society for Microbiology 
to review and possibly develop standards for training in microbio- 
logical techniques for recombinant DNA research. 
ISSUES FOR THE COMMITTEE'S CONSIDERATION 
Several commentators pointed out that the proposed revised 
Guidelines do not require an autoclave in the P3 laboratory itself, 
but only within the building. The current Guidelines provide for an 
autoclave "within the building, and preferably within the controlled 
laboratory area." In light of the concerns expressed, would the 
Committee object to retaining the language in the current Guidelines? 
B . Biological Containment 
There were a number of comments on the development of alternate 
host-vector systems, the certification process, and distribution 
of certified host-vectors. Specific suggestions were also received 
regarding each of the HV systems. On the basis of those comments, 
I recommend that the Committee consider the following: 
ISSUES FOR THE COMMITTEE'S CONSIDERATION 
• Concerning the HV1 system, objections were made to a requirement 
that the host should have "a low potential for survival in its 
natural environment." Many of the host cells that investigators 
may wish to use have no natural environment other than the 
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