11 
apply to these fungal or similar lower eukaryotic host-vector 
systems . 
"Once approved by NIH, HV1 systems may be used under P2 
containment for shotgun experiments with phages, plasmids, and 
DNA from nonpathogeni c prokaryotes and lower eukaryotes that 
do not produce polypeptide toxins (i.e., organisms that can 
be cloned into EK1 hosts under P2 conditions). Should HV2 
or HV3 systems of this type be developed and approved by NIH, 
guidelines for their use in other types of recombinant DNA 
experiments will also be established." 
IV . Roles and Responsibilities 
There was a great deal of comment directed to the relevant roles 
and responsibilities of the institutions and NIH as outlined in this 
section. Several commentators requested more information and greater 
clarification of the structure and operation of the institutional bio- 
hazards committees (to be called "institutional biosafety committees' 1 
in the revision), the function of the biological safety officer, and 
the duties of the institution. Many other comments were devoted to 
the membership and functions of the RAC and the responsibilities of 
the Director, NIH, under the proposed revisions. In light of these 
comments and my review of the administration of the Guidelines over the 
past -two years, I would like the Committee to consider the following 
suggestions : 
• that the contents of Part IV be subsumed under three general 
[512] 
