APRIL 27-28-MINUTES OF MEETING 
18 
compatibility with the amino acid sequence. Analysis with 
restriction endonucleases shows the expected pattern of fragments. 
E. A request by Dr. Carl Schildkraut to propagate characterized 
plasmids { ZMaG and ZM8G) containing cENA copies of mouse a and 
Bglobin DNA was unanimously approved at P2 + EKl. The partial 
nucleotide sequences correspond to the amino acid sequence, 
the restriction pattern is consistent, and the plasmid ENA 
hybridizes to mouse glob in ENA. 
F. A similar request by Dr. Ralph Giorno to propagate characterized 
plasmids containing mouse a and 6 glob in ENA under P2 + EKl conditions 
was also unanimously approved contingent upon either the demonstration 
that _E. coli strain N543 does not have conjugative plasmids or 
that a host which meets EKl criteria is used. 
G. Dr. John Baxter and colleagues requested permission to reduce 
containment levels from P3 + EK2 to P3 + EKl or P2 + EK2 for 
a series of clones containing all or part of the gene for rat 
growth hormone. Nucleotide sequence and restriction analysis 
data indicates correspondence with the amino acid sequence. 
The question of potential harm if the gene were to be expressed 
was raised by Dr. Hel inski. Dr. Gottesman indicated that the 
amount of growth hormone produced in E. coli would not affect 
human hormonal balance. Other evidence indicates that rat 
hormone does not cross-react with human and that growth hormone 
is totally inactive when administered orally. The RAC unanimously 
voted approval for P3 + EKl or P2 + EK2 containment levels. 
Later Dr. Gottesman made a motion to permit lowering of containment 
levels for characterized rat growth hormone clones to P2 + EKl. 
Ihe motion passed 8 to 2. 
H. A second request from Dr. Baxter to propagate characterized clones 
of human growth hormone and human scmatcmairmo tropin (related to 
growth hormone) at P2 + EKl containment levels was approved on 
the grounds that the inserts were characterized. Hie nucleotide 
sequence for amino acids 24-190 has been determined. The RAC 
unanimously voted approval of P3 + EKl or P2 + EK2 containment 
levels for these clones. 
A second motion to lower the containment requirements to P2 + EKl 
for characterized clones of human growth hormone resulted in a 
vote of six for and six against. Dr. Stetten voted no and the 
motion was defeated. 
I. A request by Dr. Howard Goodman to propagate characterized clones of 
rat insulin ENA under P2 + EKl conditions was approved by a vote of 
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