APRIL 27-28-MINUTES OF MEETING 
20 
analysis stems fran the present knowledge of the structure of 
viral genomes and their mode of replication. 
A. The Working Group recommended that mouth pipetting be prohibited 
at the Pi physical containment level. This evoked considerable 
discussion by the RAC. It was the consensus of the RAC that 
many experiments classified as Pi need not include a ban on 
mouth pipetting, and that therefore Pi in general should not 
be redefined. However, the RAC by a vote of 10 to 2 approved 
the principle that Pi with an added prohibition on mouth 
pipetting should be endorsed for designated experiments. 
B. The Working Group recommended for certain classes of experiments 
the use of E. coli K-12 with " non-mob ilizable plasmid vectors," 
i.e., a level of containment falling between EKl and EK2. 
Dr. Tbelberg pointed out that the terminology was incorrect 
and rather than speaking of "non-mob ilizable vectors" one 
should speak of "vectors certified for use in EK2 systems." 
With this change the RAC unanimously endorsed this new level 
of biological containment to be used in specific instances. 
C. It was pointed out that acceptance of the Working Group 
recommendations would remove from the current prohibited 
experiments both the use of vesicular stomatitis virus 
(a Class 3 CDC virus) , and all of the oncogenic viruses 
classified by NCI as moderate risk agents. There was 
no objection to this by the RAC. It was pointed out that 
the NCI list is outdated and was conservative. 
D. The RAC then discussed Table 1 and Table 2 of the Working 
Group Report and the accompanying text describing the table. 
This text would be inserted into the Guidelines. The inclusion 
of the tables in the Guidelines was also recoimended but not 
mandatory. The RAC made some changes in the texts and tables, 
and then unanimously adopted them. (Attachments VTII-A and 
VIII-B) . 
The effect of this is as follows: 
Section III-B-l-a-( l)-(g) , "Cloning of Viral Gencmes frcm 
Eukaryotic Cell DNA" of the proposed revised Guidelines, 
is eliminated. Section IIl-B-l-b-( 1) , "Viruses of Eukaryotes" 
is eliminated; substituted for it is the text attached 
to the Minutes as Attachment VIII-A. In section IIH-3, 
"Experiments with Eukaryotic Host-Vectors," subparts 
a-"Vertebrate Host-Vector Systems," and b-" Invertebrate 
Host-Vector Systems," are eliminated; substituted is 
text attached to the Minutes as Attachment VIII-B, which 
consists of three subparts: a-"Vertebrate Host-Vector 
Systems 1 ; b-" Invertebrate Host-Vector Systems in Which 
Insect Viruses Are Used to Propagate Other ENA Segments"; 
and c-”Plant Viral Host-Vector Systems." 
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