APRIL 2 7-2 8 -MINUTES OF MEETING 
27 
K. Cloning a Region of SV40 into E. coli K-I2 
Dr. Robert Tjian of the Oold Spring Harbor Laboratory submitted a 
request to clone DMA sequences which lie within a segment of SV40 
ENA which does not encode any known gene products but is likely 
to contain part or all of the origin of SV40 relication. It is 
proposed to insert these fragments into an EK2 host-vector system 
under P3 conditions. 
The RAC felt that the 1976 Guidelines appear to require P4-EK2 
or P3-EK3 conditions, because the segments to be inserted, 
although purified, were not cloned and, therefore, do not meet 
the requirement that the segment must be cloned prior to lowering 
the required containment to P3-EK2 conditions. However, members 
of the RAC felt that the degree of purification of the segments 
to be inserted is likely to be equivalent to that achievable by 
cloning. The RAC voted unanimously that an exception should be 
granted to conduct these experiments under P3-EK2 conditions if 
certain additional information on the degree of purification is 
available. 
L. Cloning in Salmonella typhimurium 
The RAC reviewed a request f ran a group of eleven scientists to 
permit reccmbinant ENA experiments involving the introduction of 
S . typhimunun and/or E_. coli DNA into S_. typhimurium . 
The RAC approved the S almonella experiments on the basis that it is 
their interpretation of the Guidelines that S_. typh imuriun strain 
LT2 can be considered as a host equivalent to EL coli K-12 for the 
purposes of cloning typhiumuriun or Eb coli DNA. The RAC 
then unanimously passed a motion that experiments which involve 
the transfer of S_. typnimurium DNA cloned in Eb coli back into 
S. typhimurium LT2 should be approved under P2 conditions. 
IX. WORKSHOP ON RISK ASSESSMENT OF AGRICULTURAL PATHOGENS 
Dr. Day presented the report of the Workshop on Risk Assessment of 
Agricultural Pathogens held on March 20-21, 1978. A number of the 
recommendations of the Workshop Report had already been adopted 
by the RAC earlier in the meeting. Additional changes in the 
Guidelines recommended in the Workshop Report and approved unan- 
imously by the RAC were the following: 
A. A motion to include in the Guidelines, as part of Appendix B, 
the "Hazard Classification of Plant Pathogens," as taken from 
the Workshop Report, was approved with a minor addition pro- 
posed by Dr. Day. The material to be added to Appendix B of 
the proposed revised Guidelines is attached to the Minutes as 
Attachment IX. 
[ 543 ] 
