APRIL 27-28-MINUTES OF MEETING 
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B. Section III-B-l-a-( l)-(e) on "Other Cold-Blooded Animals and 
Lower Eukaryotes." Under item 2, the first sentence in the 
proposed revised Guidelines is: "2. The remainder of the 
species in this class: P2 + EKl or Pi + EK2." Change this to: 
"2. The remainder of the species in this class, including 
plant pathogenic or symbiotic fungi that do not produce potent 
toxins: P2 + EKl or Pi + EK2." 
C. Section III-B-l-a- ( 1)- ( f ) on "Plants." The first two sentences 
in the proposed revised Guidelines are: "(f) Plants. P2 physical 
containment + an EKl host- vector or Pi + EK2. If the plant 
source carries a known pathogenic micro-organism or makes a 
potent polypeptide toxin (footnote), the containment must be 
raised to P3 physical containment + an EK2 host-vector." 
The change is to delete from the second sentence the words 
"carries a known pathogenic micro-organism or." 
D. Section III-B-3-c on "Plant host-vector systems" in the proposed 
revised Guidelines will now become Section III-B-3-d to be 
called "Plant host-vector systems other than plant viruses." 
IX. OTHER CHANGES IN THE PROPOSED REVISED GUIDELINES 
At the conclusion of the meeting, the RAC discussed other changes in 
the proposed revised Guidelines. In its deliberations, the RAC took 
into consideration the previously voted upon alterations in the Virus 
Section. 
A. Shotgun of Primate DNA into E. coli K-12 
Cne of the reasons given originally for the high containment level 
for shotgun experiments involving primate ENA into _E. coli K-12 
was the possible inadvertent cloning of viral DNA. In view of 
the adoption of the recommendations of the Virus Working Group 
to lower containment for deliberate cloning of viral DNA, the 
RAC reconsidered primate shotgun levels, and voted unanimously 
to rewrite Section III-B-l-a-( l)-(a) , as follows (although the 
exact wording was not voted upon): 
"(a) Primates. P2 physical containment + an EK2 host-vector. 
However any lowering of containment below these levels (i.e., 
for purified DNA or characterized clones) cannot be made solely 
by an institutional biosafety ccmmittee but requires NIH approval." 
[Note: It was not discussed at the RAC meeting, but additional 
text and cross references will now be put into Section III-B-l-c 
"lowering of containment levels of characterized or purified 
DNA preparations and clones"- to remind the reader that primate 
shotguns are to be treated differently.] 
[ 544 ] 
