APRIL 27-23-MINUTES OF MEETING 
29 
B . "Self-Cloning in Prokaryotic Hosts that Exchange Genetic Information 
with E. coli 
Wording to be inserted into tl»e proposed revised Guidelines to 
allow "self-cloning" experiments in prokaryotic hosts that 
exchange genetic information with E. coli , using E. coli K-12 
as an "intermediate host," at Pi physical containment was read 
to the RAC by Dr .* Gottesinan and approved unanimously. After 
the RAC meeting, the text was reworded for clarity. The text, 
to be inserted under Section III-B-2, "Experiments with other 
prokaryotic host- vectors," would read: 
"It is expected that prokaryotes that exchange genetic 
information with E. coli will be exempted from tliese 
Guidelines by appearing on the "list of exchangers" 
(see exemption iv). FOr a prokaryote which exchanges 
genetic information with E. coli but which is not on 
the list (Host A) , the following experiments may be 
carried out under Pi conditions without Host A having 
been approved as an HVl host: 
DMA from Host A may be cloned into a vector 
and propagated in E. coli K-12 under Pi 
conditions. Subsequently, this recombinant 
DNA may be returned to Host A by mobilization 
or transformation, and then may be piopagated 
in Host A under Pi conditions ." 
X. DATES OF FUTURE MEETINGS 
Hie Oommittee selected August 2-3, 1978, and October 30-31, 1978, as 
dates of future meetings. 
XI. ADJOURNMENT 
The meeting was adjourned at 4:00. p.m., April 28, 1978. 
Respectfully submitted, 
Executive Secretary 
[ 545 ] 
