sentence of paragraph 100 to read: 
"Decontamination of run-off water is not 
necessarily required." 
She said she agreed with this and proposed the language be 
changed to reflect this. Also, the IBA proposed doing away with 
paragraph 91c in its entirety which relates to biological signs 
for BL1-P containment. Dr. Fedoroff and Dr. Tolin agreed that 
this was an acceptable amendment to the proposal. 
Dr. Vidaver said she had an addition to paragraph 106a. She 
suggested a sentence be added to say that, "Soil beds are also 
acceptable unless propagules of experimental organisms are 
readily disseminated through soil." She said there could be some 
question about doing such an experiment with the current wording 
of 106a. As long as they remained confined, she saw no reason to 
have soil beds inside greenhouses. She also said it had been 
suggested that in paragraph 50 the beginning of the second 
sentence be changed from the wording, "Recognition as to 
whether..." to "Determination of whether or not...." Dr. 
Fedoroff agreed with both changes. 
Dr. Walters asked regarding the last sentence in paragraph 88 on 
page 26 whether the "potential consequences" referred to were 
ecological consequences or administrative consequences. After 
discussion, it was determined to remove the entire last sentence 
from paragraph 88. 
Dr. Korwek said that there were still some inconsistencies in 
paragraphs 100 and 114 referring to the use of autoclaves for 
sterilization. Dr. Tolin replied that at BL1-P organisms could 
be killed by means other than autoclaving; and it leaves it to 
the investigator to determine the best method for inactivating 
organisms. Dr. Korwek said in that case the terminology "render- 
ing biologically inactive" was redundant. Dr. Pirone said he 
felt it was clear without the use of the word "autoclave" that 
any means to biologically inactivate an organism was all that was 
needed. 
Dr. Fedoroff said the original document before translation into 
the language of the NIH Guidelines simply said: 
"Experimental organisms are rendered 
biologically inactive by appropriate method 
before disposal." 
She asked Dr. Gartland to reword this in paragraphs 100 and 114 
to ensure the original intent was clear. 
Dr. Morris Levin of the Environmental Protection Agency said he 
felt the proposal was well thought out and constructive. He felt 
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