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Federal Register / Vol. 52. No. 154 / Tuesday. August 11. 1987 / Notices 
should be given to preventing spread by 
that route. In the absence of specific 
knowledge of a particular route of 
transmission, all potential means of 
horizontal transmission, including 
arthropods, contaminated bedding, or 
animal waste should be prevented.'* 
238. "Appendix Q-H-C-l-c. Eating, 
drinking, smoking, and applying 
cosmetics are not permitted in the work 
area." 
239. "Appendix Q-ll-C-l-d. If 
experiments involving other organisms 
which require lower levels of 
containment are to be conducted in the 
same area concurrently with 
experiments requiring BL3-N 
containment, they shall be conducted in 
accordance with BL3-N practices." 
240. "Appendix Q-U-C-l-e. Persons 
shall wash their hands after handling 
materials involving organisms 
containing recombinant DNA molecules 
and animals and when they leave the 
animal area." 
241. "Appendix Q-II-C-l-f. Animal 
holding areas shall be decontaminated 
at least once a day and after any spill of 
viable material." 
242. "Appendix Q-U-C-l-g. All 
procedures shall be performed carefully 
so as to minimize the creation of 
aerosols." 
243. "Appendix Q-U-C-2. Special 
Practices — BL3-N. " 
244. "Appendix Q-II-C-2-a. The 
containment areas will be locked." 
245. "Appendix Q-U-C-2-b. The area 
will be patrolled or monitored at 
frequent interv als." 
246. "Appendix Q-Il-C-2-c. A double 
barrier shall be provided to separate 
male(s) and female(s) animals unless 
reproductive studies are part of the 
experiment or other measures are taken 
that avoid reproductive transmission. 
Reproductive incapacitation can be 
utilized if needed." 
247. "Appendix Q-lI-C-2-d. The 
containment building will be controlled 
and have a lockable access." 
248. "Appendix Q-ll-C-2-e. All 
animals must be euthanized at the end 
of their experimental usefulness and the 
carcasses shall be decontaminated 
before disposal in an approved manner. 
Documents regarding animal 
experimental use and disposal shall be 
maintained in o permanent log book." 
249. "Appendix Q-II-C-2-f. The 
director shall establish policies and 
procedures whereby only persons who 
have been advised of the potential 
hazard and who meet any specific entry 
requirements (e g . vaccination) and who 
comply with all entry requirements may 
enter the laboratory or animal rooms." 
250 "Appendix Q-lI-C-2-g. When the 
animal research requires special 
provisions for entry (e g., vaccination), a 
hazard warning sign incorporating the 
universal biohazard symbol shall be 
posted on all access doors to the animal 
work area. The hazard warning sign 
shall identify the agent, animal species, 
list the name and telephone number of 
the director, or other responsible 
person(s) and indicate the special 
requirement(s) for entering the 
laboratory. 
251. "Appendix Q-U-C-2-h. Full 
protective clothing that protects the 
individual (e g., scrub suits, coveralls, 
uniforms) 6hall be worn in the animal 
area. Clothing shall not be worn outside 
the animal containment zone, and it 
shall be decontaminated before being 
laundered. 
252. "Appendix Q-U-C-2-i. Animals 
of the same or different species not 
involved in the work being performed 
shall not be permitted in the animal 
area." 
253. "Appendix Q— II— C— 2— j- Special 
care shall be taken to avoid skin 
contamination with microorganisms 
containing recombinant DNA. 
Impervious and/or protective gloves 
shall be worn when handling 
experimental animals and when skin 
contact with the infectious agent is 
unavoidable. 
254. "Appendix Q-Il-C-2-k. 
Hypodermic needles and syringes shall 
be used only for parenteral injection and 
aspiration of fluids from laboratory 
animals and diaphragm bottles. Only 
needle-locking syringes or disposable 
syringe-needle units (i.e.. needle is 
integral to the syringe) shall be used for 
the injection or aspiration of fluids 
containing organisms that contain 
recombinant DNA molecules. Extreme 
caution shall be used when handling 
needles and syringes to avoid 
autoinoculation and the generation of 
aerosols during use and disposal. 
Needles shall not be bent, sheared, 
replaced in the needle sheath or guard 
or removed from the syringe following 
use The needle and sy'ringe shall be 
promptly placed in a puncture-resistant 
container and decontaminated, 
preferably by autoclaving, before 
discard or reuse." 
255. "Appendix Q— II— C— 2— 1. All 
incidences involving spills and 
accidents which result in environmental 
release or exposures of animals or 
laboratory workers to organisms 
containing recombinant DNA molecules 
shall be immediately reported to the 
laboratory director. Medical evaluation, 
surveillance, and treatment shall be 
provided as appropriate and written 
records shall be maintained. If 
necessary , the area will be 
appropriately decontaminated 
256. "Appendix Q-Il-C-2-m. When 
appropriate, and giving consideration to 
the agent(s) handled, baseline serum 
samples shall be collected and stored 
for animal care and other at-risk 
personnel. Additional serum specimens 
may be collected periodically depending 
on the agents handled or the function of 
the facility." 
257. "Appendix Q-lI-C-2-n. A 
biosafety manual shall be prepared or 
adopted. Personnel shall be advised of 
special hazards and shall be required to 
read instructions on practices and 
procedures and to follow them.” 
258. "Appendix Q-II-C-2-o. Biological 
materials to be removed from the animal 
containment laboratory in a viable or 
intact state shall be transferred to a 
nonbreakable. sealed primary container 
and then enclosed in an nonbreakable, 
sealed secondary container. All 
containers, primary and secondary, shall 
be disinfected before removal from the 
facility. Advance approval for transfer 
of material must be obtained from the 
director. Such packages containing 
viable agents can only be opened in 
another BL3-N facility unless the agent 
is biologically inactivated or is 
nonreproductive. Special safety testing, 
decontamination procedures, and IBC 
approval are required to move agents or 
tissue/organ specimens from a BL3-N 
facility to one with a lower containment 
classification." 
259. “Appendix Q-II-C-2-p. Animal 
room doors and gates or other closures 
shall be kept closed when experiments 
are in progress." 
2G0 "Appendix Q-lI-C-2-q. The work 
surfaces of containing equipment shall 
be decontaminated when work with 
organisms containing recombinant DNA 
molecules is Finished. Where feasible, 
plastic-backed paper toweling shall be 
used on nonporous work surfaces to 
facilitate clean-up." 
261. "Appendix Q-lI-C-2-r. Molded 
surgical masks or respirators shall be 
worn in rooms containing experimental 
animals." 
262. "Appendix Q-Il-C-3, Special 
Animal Facilities — BL3-N . " 
263. "Appendix Q-II-C-3-a. All 
animals shall be contained within an 
enclosed structure (animal room or 
equivalent) to avoid the possibility of 
theft or unintentional release and avoid 
access of arthropods. The special 
provision to avoid the entry or escape of 
arthropods from the animal areas may 
be waived if the agent in use in known 
not to be transmitted by arthropods." 
264. "Appendix Q-Il-C-3-b. The 
interior surfaces of walls, floors, and 
ceilings shall be impervious to water 
and resistant to acids, alkalis, organic 
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