29806 
Federal Register / Vol. 52. No. 154 / Tuesday. August 11, 1937 / Notices 
BL3-P level provided all work is 
conducted in accordance with BL3-P 
greenhouse practices. 
121. "Appendix P-U-C-2. Facilities — 
BL3-P:' 
122. "The term 'greenhouse' refers to a 
permanent structure with walls, roof, 
and floor designed and utilized 
principally for growing plants in a 
controlled and protected environment. 
Walls and roof are usually constructed 
of transparent or translucent material to 
allow passage of sunlight for plant 
growth. The term ‘greenhouse facility" 
includes hallways and headhouse areas 
immediately contiguous to the actual 
greenhouse rooms or compartments for 
growing plants, and is considered part of 
the confinement area.” 
123. "The floor of the greenhouse is of 
concrete or other impervious material 
with provision for collection and 
decontamination of liquid mn-off.” 
124. "Windows are closed and sealed. 
All glazing is resistant to breakage. The 
need to maintain negative pressure 
should be considered when constructing 
or renovating the greenhouse." 
125. "The greenhouse is a permanent, 
closed, self-contained structure with a 
continuous covering which is separated 
from areas open to unrestricted traffic 
flow. The greenhouse has impervious 
floors, such as concrete, with provision 
for collection and decontamination of 
liquid nm-off. The need to maintain 
negative pressure should be considered 
when constructing or renovating the 
greenhouse. Passage through two sets of 
lockable self-closing doors is the basic 
requirement for entry into the 
greenhouse." 
120. The greenhouse facility is 
surrounded by a security fence or is 
protected by an equivalent means of 
security." 
127. "The internal walls, ceilings, and 
floors are resistant to penetration by 
liquids and chemicals to facilitate 
cleaning and decontamination of the 
area. All penetrations in these structures 
and surfaces, such as plumbing and 
utilities, are sealed." 
128. "It is recommended that bench 
tops and other work surfaces have 
seamless surfaces Impervious to water 
and resistant to acida. alkahs. organic 
solvents, and moderate beat" 
12ft. "A hand-washing facility is 
located near the greenhouse exit door." 
130. "An autoclave for 
decontaminating materials is available 
within the facility. A double door 
autoclave for decontaminating materials 
passing out of the facility is 
recommended, but not required." 
131. "Vacuum lines are protected with 
high efficiency particulate air (HEPA) or 
equivalent filters and liquid disinfectant 
traps." 
132. "An individual supply and 
exhaust air ventilation system is 
provided. The system maintains 
pressure differentials and directional 
airflow as required to assure flows 
inward from areas outside the 
greenhouse." 
133a. "The exhaust air from the 
facility is filtered through HEPA filters 
and discharged to the outside. The filter 
chambers are designed to allow in situ 
decontamination before fillers are 
removed and to facilitate certification 
testing after they are replaced. Air 
supply filters shall be 80-85% average 
efficiency by the ASHRAE Standard 52- 
68 test method using atmosphere dust. 
Air supply fans are equipped with 
backflow damper that will close when 
the air supply fan is off. The supply and 
exhaust airflow is interlocked to assure 
inward (or zero) airflow at all times." 
133b. The BL3-P greenhouse 
containment requirement can be met by 
a growth chamber or growth room 
within a building, provided that the 
location, access, airflow patterns, and 
provisions for decontamination of 
experimental materials and supplies 
meet the intent of the foregoing clauses 
of the present section." 
134. "Appendix P-ll-D. BLA-P 
( Biosafety Level 4 — plants). ” 
135. "Appendix P-Il-D-1. Standard 
Practices — BL4-P . " 
138. “Only persons whose presence in 
the greenhouse is required for program 
or support purposes are authorized to 
enter. The principal investigator has the 
final responsibility for assessing each 
circumstance and determining who may 
enter or work in the greenhouse during 
experiments. Access to the facility is 
limited by means of secure, locked 
doors; accessibility is managed by the 
greenhouse director, local biosafety 
officer, or other person responsible for 
physical security of the facility. Before 
entering, persons are advised of the 
potential environmental hazards and 
instructed on appropriate safeguards for 
ensuring environmental safety. Persons 
authorized to enter the facility comply 
with the instructions and all other 
applicable entry and exit procedures. A 
record of all personnel indies ting the 
date and time of each entry and exit is 
maintained." 
137. "Personnel enter and leave the 
facility only through the dothing change 
and shower rooms. Personnel shower 
each time they leave the fadlity. 
Personnel uee the airlocks to enter or 
leave the laboratory only in an 
emergency. During an emergency, every 
reasonable effort should be made to 
prevent the possible transport of viable 
propagules from containment." 
138. "A greenhouse operations manual 
is prepared and adopted. The manual 
includes contingency plans to be 
implemented in the event of the 
unintentional release of experimental 
organisms. Personnel are required to 
read instructions on practices and 
procedures and to follow them." 
139. "A warning sign incorporating the 
universal biohazard symbol is posted on 
all access doors. The sign identifies the 
plants, microorganisms, and animals in 
use. lists the name of the prindpal 
investigator, greenhouse director, or 
other responsible person(s) and 
indicates any special requirements for 
entering the area." 
140. "Experimental materials to be 
brought into or to be removed from the 
greenhouse in a viable or intact state are 
put in a nonbreakable, sealed primary 
container and then enclosed In a 
nonbreakable. sealed secondary 
container which is removed from the 
facility through a chemical disinfectant 
or fumigation chamber, or an airlock 
designed for this purpose. A log is kept 
of all experimental material entering 
and leaving the greenhouse." 
141. “Supplies and materials needed 
in the facility are brought in by way of 
the double-doored autoclave, fumigation 
chamber, or airlock which is 
appropriately decontaminated between 
each use. After securing the outer doors, 
personnel within the facility retrieve the 
materials by opening the interior door of 
the autoclave, fumigation chamber, or 
airlock. These doors are secured after 
materials are brought into the facility." 
142. "No materials, except for 
experimental materials that are to 
remain in a viable or intact state, are 
removed from the maximum 
containment greenhouse unless they 
have been autoclaved. Equipment or 
material which might be damaged by 
high temperatures or steam is 
decontaminated by other methods such 
as gaseous or vapor methods in an 
airlock or chamber designed for this 
purpose." 
143. "Water that comes in contact 
with experimental material treated with 
microorganisms (e.g.. run-off from 
watering plants) is collected and 
decontaminated before disposal." 
144. "Standard microbiological 
procedures are followed for 
decontamination of contaminated 
equipment Hnd materials. Spray or 
liquid waste or rinse water from 
containers used to apply the 
experimental microorganisms shall be 
decontaminated before disposal." 
Recombinant DNA Research. Volume 13 
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