Federal Register / Vol. 52, No. 154 / Tuesday, August 11. 1987 / Notices 
28805 
practices manual is prepared to describe 
these practices. It should advise 
personnel of potential consequences if 
practices are not followed and outline 
contingency plans in the event 
containment loss results in release of 
organisms with recognized potential for 
serious detrimental impact." 
99. “A log is kept to experimental 
plants, microorganisms, or small 
animals that are brought into or 
removed from the greenhouse facility. 
Materials containing experimental 
microorganisms that are brought Into or 
removed from the greenhouse facility in 
a viable or intact state are transferred in 
a closed nonbreakable container.” 
100. “Experimental organisms are 
rendered biologically inactive by 
appropriate methods before disposal 
outside of the greenhouse facility. 
Decontamination of run-off water is not 
required. If part of the greenhouse is 
gravel or similar material appropriate 
treatments should be made periodically 
to eliminate any organisms potentially 
entrapped by the gravel." 
101. “Insects and other motile 
macroorganisms are housed in 
appropriate cages. If microorganisms, 
such as flying insects or nematodes, are 
released within the greenhouse, 
precautions are taken to minimize 
escape from the facility.” 
102. “A program is utilized to control 
undesired species, such as weed, rodent 
or insect pests and pathogens, by 
methods appropriate to the organisms 
and In accordance with applicable State 
and Federal laws." 
103a. “A sign incorporating the 
universal biohazard symbol and the 
name of the recombinant DNA-modified 
organism is posted on greenhouse 
access doors. The presence of organisms 
having a recognized potential for serious 
detrimental impacts to managed or 
natural ecosystems should also be 
indicated on these signs when used in 
the greenhouse." 
103b. "Experiments involving other 
organisms which require a containment 
level lower than BL2-P may be 
conducted in the same greenhouse 
concurrently with those requiring the 
BL2-P level provided all work is 
conducted in accordance with BL2-P 
greenhouse practices." 
104. "Appendix P-II-B-2. Facilities — 
BL2-P." 
105. “The term 'greenhouse' refers to a 
permanent structure with walls, roof, 
and floor designed and utilized 
principally for growing plants in a 
controlled and protected environment. 
Walls and roof are usually constructed 
of transparent or translucent material to 
allow passage of sunlight for plant 
growth." 
106a. “A greenhouse floor of an 
impervious material such as concrete la 
recommended, but gravel or other 
porous material under benches is 
acceptable unless propagules of 
experimental organisms are readily 
disseminated through soil." 
106b. "Windows and other openings 
in the walls and roof of the greenhouse 
compartments may be open for 
ventilation as needed for proper 
operation if fitted with No. 30 mesh (or 
finer) fly screens to exclude small flying 
animals (e.g., insects, birds). No special 
barrier to contain pollen or 
microorganisms is required." 
107. “If intake fans are used, measures 
must be taken to minimize the ingress of 
insects. Louvers or fans shall be 
constructed so as not to open unless the 
fan is in operation.” 
108. “An autoclave for treatment of 
contaminated greenhouse materials Is 
available in the facility." 
109. “BL2-P greenhouse containment 
requirements can be satisfied by using a 
growth chamber or growth room within 
a building, provided that the external 
physical structure limits access and 
escape of macroorganisms in a manner 
that satisfies the intent of the foregoing 
clauses.” 
110. “Appendix P-H-C, BL3-P 
(Biosafety Level 3— plants)." 
111. “Appendix P-H-C-1, Standard 
Practices — BL3-P . " 
112a. “Access to the greenhouse is 
restricted to Individuals whose presence 
is required for program or support 
purposes and are authorized to enter. 
The principal investigator has the final 
responsibility for assessing each 
circumstance and determining the 
individuals to be authorized.” 
112b. "Personnel are required to read 
instructions on BL3-P practices and to 
follow them." 
113a. "All practices are in accordance 
with practices appropriate to the 
experimental organism(s). A greenhouse 
practices manual is prepared to describe 
these practices. It should advise 
personnel of potential consequences if 
practices are not followed and outline 
contingency plans in the event 
containment loss results in the release 
of organisms with recognized potential 
for serious detrimental impact" 
113b. “A log is kept of experimental 
plants, microorganisms, or small 
animals that are brought into or 
removed from the greenhouse facility. 
Materials containing experimental 
microorganisms to be brought into or 
removed from the greenhouse facility in 
a viable or intact state are transferred in 
a nonbreakable sealed secondary 
container. The surface of the secondary 
container is decontaminated prior to 
removal from the greenhouse by passage 
through a chemical disinfectant or 
fumigation chamber, or by an 
alternative procedure demonstrated to 
be effective against the experimental 
organism. If at the time of transfer the 
same plant species, hosts, or vectors are 
present within the effective 
dissemination distance of propagules of 
the experimental organism(s)." 
114. "All experimental materials, 
except those that are to remain in a 
viable or intact state for experimental 
purposes, are sterilized in an autoclave 
or rendered biologically inactive by an 
alternate effective method before 
removal from and disposal outside of 
the greenhouse facility. This includes 
water that comes in contact with 
experimental microorganisms or with 
material exposed to them, as well as all 
contaminated equipment and supplies." 
115. “A program is implemented to 
control undesired species, such as weed, 
rodent, or insect pests and pathogens, 
by methods appropriate to the 
organisms and in accordance with 
applicable State and Federal laws.” 
116. "Insects and other motile 
macroorganisms are housed in cages 
appropriate to the organisms and 
experiments are conducted within cages 
designed to contain the motile 
organisms.” 
117. “A sign incorporating the 
universal biohazard symbol and the 
name of the recombinant DNA-modified 
organism is posted on greenhouse 
access doors. The presence of organisms 
having a recognized potential for serious 
detrimental impacts to managed or 
natural ecosystems should also be 
indicated on these signs when used in 
the greenhouse." 
118. All procedures are performed 
carefully to minimize the creation of 
aerosols and excessive splashing of 
potting material/soil during watering, 
transplanting and all experimental 
manipulations.” 
119. Disposable clothing or solid front 
or wrap-around gown, scrub suit, or 
other appropriate clothing is worn in the 
greenhouse if deemed necessary by the 
principal investigator because of 
potential dissemination of the 
experimental microorganism(s). and 
removed upon exit from the greenhouse. 
The protective clothing worn in the 
greenhouse is decontaminated prior to 
laundering or disposal. Personnel 
thoroughly wash their hands upon 
exiting the greenhouse." 
120. Experiments involving other 
organisms which require a containment 
level lower than BL3-P may be 
conducted in the same greenhouse 
concurrently with those requiring the 
[ 26 ] 
Recombinant DNA Research, Volume 13 
