The working group recommended that the title of Section III-B-4 be amended 
to read as follows: 
"Experiments Involving Whole Animals, Including Transgenic Animals." 
D. On September 21, 1987, the RAC recommended that paragraph 34 as published 
for comment in the Federal Register of August 11, 1987, be incorporated 
into Section III-B-4 of the NIH Guidelines as follows: 
"This section ca/ers experiments involving whole animals, both those 
in which the animal's genome has been altered by recombinant DNA tech- 
niques and experiments involving viable recambinant-ENA-modified micro- 
organisms tested on whole animals. For the latter, other than viruses 
which are only vertically transmitted, the experiments may not be 
carried out at BL1-N containment; a minimum containment of BLl or 
BL2-N is required." 
The working group recanmended that this section be amended to read as follows 
"This section covers experiments involving whole animals, both those 
in which the animal's genane has been altered by stable introduction 
of DNA into the germ line (transgenic animals) and experiments involving 
viable reccmbinant-ENA-modified microorgan isms tested on whole animals. 
For the latter, other than viruses vdiich are only vertically transmitted, 
the experiments may not be carried out at BLl-N containment; a minimum 
containment of BLl or BL2-N is required. 
" Caution: Special care should be used in the evaluation of containment 
conditions for some experiments with transgenic animals. For example, 
such experiments might lead to the creation of novel mechanisms or 
increased transmission of a recombinant pathogen or production of 
undesirable traits in the host animal. In such cases, serious consid- 
eration should be given to increasing the containment conditions." 
E. On September 21, 1987, the RAC recommended that a modified paragraph 37 as 
published for comment in the Federal Register of August 11, 1987, be incor- 
porated as Section III-B-4-a of the NIH Guidelines as follows: 
"Recombinant DNA, or RNA molecules derived therefrom, from any source 
except for a eukaryotic viral genome may be transferred to any non-human 
vertebrate or any invertebrate organism and propagated under conditions 
of physical containment comparable to BLl or BLl-N and appropriate to 
the organism under study [2] . Animals containing sequences from viral 
vectors, if the sequences do not lead to transmissible infection either 
directly or indirectly as a result of complementation or recombination 
in animals, may be propagated under conditions of physical containment 
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Recombinant DNA Research, Volume 13 
