comparable to BL1 or BL1-N and appropriate to the organism under study. 
For experiments involving recombinant ENA modified Class 2, 3, 4, or 5 
organisms (1) using whole animals, see Section III-B-1." 
The working group recommended that this section be amended to read as follows 
"Recombinant DNA, or DNA or R1& molecules derived therefrom, from any 
source except for a eukaryotic viral genome may be transferred to any 
non-human vertebrate or any invertebrate organism and propagated under 
conditions of physical contairment comparable to BL1 or BL1-N and 
appropriate to the organism under study [2]. Animals containing sequence 
from viral vectors, if the sequences do not lead to transmissible 
infection either directly or indirectly as a result of complementation 
or recombination in animals, nay be propagated under conditions of 
physical containment comparable to BL1 or BLl-N and appropriate to the 
organism under study. Experiments involving the introduction of other 
sequences from eukaryotic viral gencmes into animals are covered under 
III-B-4-b. For experiments involving recombinant ENA modified Class 
2, 3, 4, or 5 organ isns (1) using whole animals, see Section III-B-1." 
F. Cn September 21, 1987, the RAC recommended that paragraph 41 as published 
for comment in the Federal Register of August 11, 1987, be incorporated as 
Section III-B-4-b of the NIH Guidelines, as follows: 
"For experiments involving whole animals and not covered by Section 
III-B-1 or Section III-B-4-a, the appropriate contairment will be 
determined by the IBC (22,23)." 
The working group recommended that this section be amended to read as follows 
"For experiments involving recombinant DNA, or DNA or RNA derived there- 
from, involving whole animals, including transgenic animals, and not 
covered by Section III-B-1 or Section III-B-4-a, the appropriate contain- 
ment will be determined by the IBC (22)." 
Dr. Singer asked whether limits should be placed on numbers of animals in the 
cautionary note. Dr. Rapp responded that numbers would depend entirely on the 
animal species under consideration. Dr. Gottesman said that IBCs could be 
advised that scale is an option. 
Dr. Rapp recommended that the changes, vhen adopted, be highlighted in the 
Recombinant DNA Technical Bulletin. 
Recombinant DNA Research, Volume 13 
[ 119 ) 
