by sexual reproduction). Procedures, practices, and 
facilities follow classical methods of avoiding genetic 
exchange between animals." 
The working group recommended that "(transgenic animals)" be 
deleted from this paragraph, but to define "transgenic animal" 
later as "the stable introduction of DNA into the germ line." 
In recommendation B, the working group recommended adding the 
following new sentence at the beginning of Section III-B-3, 
before the paragraph entitled "Caution": 
"Experiments involving the introduction of eukaryotic 
viral genomes into the germ line of animals are covered 
in Section III-B-4." 
Dr. Gottesman said this was introductory information designed to 
aid IBCs in reviewing experimental protocols in which there is 
possibility of transmission. 
In recommendation C, the working group recommended changing the 
title of Section III-B-4 from "Recombinant DNA Experiments 
Involving Whole Animals," to "Experiments Involving Whole 
Animals, Including Transgenic Animals." Dr. Gottesman said this 
was merely a change in title to aid IBCs in finding information 
and did not affect the substance of the NIH Guidelines. 
In recommendation D, the working group recommended the following 
wording be incorporated into Section III-B-4 of the NIH 
Guidelines : 
"This section covers experiments involving whole 
animals , both those in which the animal ' s genome has 
been altered by stable introduction of DNA into the 
germ line (transgenic animals) and experiments 
involving viable recombinant-DNA-modif ied 
microorganisms tested on whole animals. For the 
latter, other than viruses which are only vertically 
transmitted, the experiments may not be carried out at 
BL1-N containment; a minimum containment of BL1 or 
BL2-N is required. 
" Caution: Special care should be used in the 
evaluation of containment conditions for some 
experiments with transgenic animals. For example, such 
experiments might lead to the creation of novel 
mechanisms or increased transmission of a recombinant 
pathogen or production of undesirable traits in the 
host animal. In such cases, serious consideration 
should be given to increasing the containment 
conditions . " 
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Recombinant DNA Research, Volume 13 
