Dr. Gottesman said this is the change which has been the subject 
of much discussion today and that suggestions have included 
altering the first sentence by some means to suggest that the 
introduction of DNA is performed by artificial means rather than 
natural breeding techniques. She added the cautionary paragraph 
is providing further guidance to IBCs and investigators. 
In recommendation E, the working group recommended a modification 
to Section III-B-4-a of the NIH Guidelines, to read as follows: 
"Recombinant DNA, or DNA or RNA molecules derived 
therefrom, from any source except for a eukaryotic 
viral genome may be transferred to any non-human 
vertebrate or any invertebrate organism and propagated 
under conditions of physical containment comparable to 
BL1 or BL1-N and appropriate to the organism under 
study [2]. Animals containing sequences from viral 
vectors, if the sequences do not lead to transmissible 
infection either directly or indirectly as a result of 
complementation or recombination in animals, may be 
propagated under conditions of physical containment 
comparable to BL1 or BL1-N and appropriate to the 
organism under study. Experiments involving the 
introduction of other sequences from eukaryotic viral 
genomes into animals are covered under III-B-4-b. For 
experiments involving recombinant DNA modified Class 2, 
3, 4, or 5 organisms (1) using whole animals, see 
Section III-B-1." 
Dr. Gottesman said this sets containment for a whole class of 
experiments and supersedes a change made last September to this 
same section which was an attempt to avoid the use of the phrase 
"greater than two-thirds of the eukaryotic viral genome." 
In recommendation F, the working group recommended Section III-B- 
4-b of the NIH Guidelines be modified to read as follows: 
"For experiments involving recombinant DNA, or DNA or 
RNA derived therefrom, involving whole animals, 
including transgenic animals, and not covered by 
Section III-B-1 or Section III-B-4-a, the appropriate 
containment will be determined by the IBC (22)." 
Dr. Gottesman said this closed a loophole whereby an investigator 
could cut the DNA out of the vector and argue the experiment did 
not involve recombinant DNA. 
Dr. Davis said he would accept that there is no other body in a 
better position than the RAC to offer guidelines for work on 
transgenic animals, that work is currently taking place on 
transgenic animals and guidelines are necessary. He suggested 
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