Dr. Gottesman suggested no changes be made to Section III-B-5, 
but to revise the wording of Appendix K-I of the NIH Guidelines 
to read: 
"The selection of the physical containment level 
requirement is based on the containment guidelines as 
established in Part III of the Guidelines. For 
purposes of large-scale research or production, three 
physical containment levels are established. These are 
referred to as BL1-LS, BL2-LS, and BL3-LS. The BL1-LS 
level of physical containment is recommended for large- 
scale research or production of viable organisms 
containing recombinant DNA molecules which require BL1 
containment at the laboratory scale. For large-scale 
fermentation experiments involving non-pathogenic and 
non-toxicogenic recombinant strains of host organisms 
having an extended history of safe industrial use, the 
IBC may consider suggesting containment consistent with 
good industrial practice rather than using 
BL1-LS . " 
Dr. Gottesman said this would add flexibility. It would remove 
the requirement for BL1-LS and make it a recommendation, thereby 
putting more flexibility in the hands of the IBC. 
Dr. Gellert moved Dr. Gottesman' s proposed revision to Appendix 
K-I of the NIH Guidelines be adopted. Dr. Pramer seconded the 
motion. 
Dr. Miller asked what BL1-LS was being recommended for. Dr. 
Gottesman replied it would be recommended for experiments 
requiring BL1 containment at the laboratory scale. Dr. Miller 
suggested deleting the sentence recommending BL1-LS for 
experiments requiring BL1 containment at the laboratory scale 
because he felt it was negated in the next sentence. Further, he 
said he would incorporate some of the language of the Federal 
Register notice of June 26, 1986, to say, "For recombinant 
DNA-containing organisms meeting certain criteria, appropriate 
large-scale containment will be no greater than that for the host 
organism unmodified by recombinant DNA techniques." 
Mr. Mannix suggested breaking Appendix K-I into K-I-a and K-I-b. 
Appendix K-I-a would be prefaced by "Except as in K-I-b...." and 
would read as the current K-I, but changing the word "required" 
to "recommended." Appendix K-I-b would consist of the proposed 
language before the committee for large-scale fermentation and 
read, "For large-scale fermentation experiments involving 
non-pathogenic and non-toxicogenic recombinant strains of host 
organisms having an extended history of safe industrial use the 
appropriate physical containment conditions need be no greater 
than those for the host organism unmodified by recombinant DNA 
Recombinant DNA Research, Volume 13 
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