Table 1 
MOUSE TIL INFECTION: 
Variables tested without success. 
Vectors 
6 amphotropic and ecotropic vectors known to infect mouse cells 
TIL 
5 Different TIL lines were repeatedly tested with each vector 
Polycations 
A range of concentrations of polybrene and protamine were used 
Method of Infection 
6 Experienced investigators tried using both co-cultivation for 
24 hr to 7 days and repeated supernatant infections at MOI 
ranging from 1 to 20. Fresh tumors, whole spleen, short term 
cultured TIL and restimulated TIL were tested 
Selection Conditions 
A range of selection conditions using 0.1 to 1 .0 mg/ml G418 in both 
continuous and intermittant exposure protocols were tested 
Recombinant DNA Research, Volume 13 
[ 219 ] 
