Dr. William J. Gartland, Jr. 
September 10, 1987 
Paragraph 100 
We recommend changing the wording of the second sentence to: 
"Decontamination of run-off water is not necessarily required." IBA 
believes that the present wording is inconsistent with the 
requirement to inactivate experimental organisms. We would point out 
that under certain circumstances run-off water may contain 
experimental organisms that would require decontamination. 
Paragraph 108 
We recommend changing the wording to: "An autoclave for 
treatment of contaminated greenhouse materials is available." This 
wording is consistent with the present NIH Guidelines (see Appendix 
G-II-B-4-f). Placing an autoclave in a BL2-P greenhouse is an 
unwarranted burden. The investigator should be permitted to 
transport materials that require autoclaving from the greenhouse to a 
laboratory in appropriate containers. We would point out that 
conditions for transport in and out of the greenhouse are specified 
in Paragraph 99. 
IBA hopes that these comments are useful to NIH-RAC as they 
deliberate on these matters. 
Sincerely, 
Q — * 
AG/th 
Recombinant DNA Research, Volume 13 
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